Krausz Aimee E, Adler Brandon L, Landriscina Angelo, Rosen Jamie M, Musaev Tagai, Nosanchuk Joshua D, Friedman Adam J
Division of Dermatology, Department of Medicine, Montefiore Medical Center, Albert Einstein College of Medicine, 111 E. 210th Street, Bronx, New York, 10467, USA.
Arch Dermatol Res. 2015 Sep;307(7):583-94. doi: 10.1007/s00403-015-1559-x. Epub 2015 Mar 21.
Macrophages play a fundamental role in wound healing; therefore, employing a strategy that enhances macrophage recruitment would be ideal. It was previously suggested that the mechanism by which Biafine topical emulsion improves wound healing is via enhanced macrophage infiltration into the wound bed. The purpose of this study was to confirm this observation through gross and histologic assessments of wound healing using murine full-thickness excisional and burn wound models, and compare to common standards, Vaseline and silver sulfadiazine (SSD). Full-thickness excisional and burn wounds were created on two groups of 60 mice. In the excisional arm, mice were divided into untreated control, Biafine, and Vaseline groups. In the burn arm, mice were divided into untreated control, Biafine, and SSD groups. Daily treatments were administered and healing was measured over time. Wound tissue was excised and stained to appropriately visualize morphology, collagen, macrophages, and neutrophils. Collagen deposition was measured and cell counts were performed. Biafine enhanced wound healing in murine full-thickness excisional and burn wounds compared to control, and surpassed Vaseline and SSD in respective wound types. Biafine treatment accelerated wound closure clinically, with greater epidermal/dermal maturity, granulation tissue formation, and collagen quality and arrangement compared to other groups histologically. Biafine application was associated with greater macrophage and lower neutrophil infiltration at earlier stages of healing when compared to other study groups. In conclusion, Biafine can be considered an alternative topical therapy for full-thickness excisional and burn wounds, owing to its advantageous biologically based wound healing properties.
巨噬细胞在伤口愈合中发挥着重要作用；因此，采用一种能增强巨噬细胞募集的策略将是理想的。此前有研究表明，比亚芬（Biafine）外用乳剂改善伤口愈合的机制是通过增强巨噬细胞向伤口床的浸润。本研究的目的是通过使用小鼠全层切除和烧伤伤口模型对伤口愈合进行大体和组织学评估来证实这一观察结果，并与凡士林和磺胺嘧啶银（SSD）等常用标准进行比较。在两组共60只小鼠身上制造全层切除和烧伤伤口。在切除伤口组，小鼠被分为未治疗对照组、比亚芬组和凡士林组。在烧伤伤口组，小鼠被分为未治疗对照组、比亚芬组和SSD组。每天进行治疗，并随时间测量愈合情况。切除伤口组织并进行染色，以适当观察其形态、胶原蛋白、巨噬细胞和中性粒细胞。测量胶原蛋白沉积并进行细胞计数。与对照组相比，比亚芬可促进小鼠全层切除和烧伤伤口的愈合，并且在各自的伤口类型中超过了凡士林和SSD。比亚芬治疗在临床上加速了伤口闭合，与其他组相比，在组织学上具有更高的表皮/真皮成熟度、肉芽组织形成以及更好的胶原蛋白质量和排列。与其他研究组相比，在愈合早期，应用比亚芬与更多的巨噬细胞浸润和更少的中性粒细胞浸润相关。总之，由于其基于生物学的有利伤口愈合特性，比亚芬可被视为全层切除和烧伤伤口的一种替代局部治疗方法。
