银纳米颗粒增强了一种用于测定6-巯基嘌呤的新型TCPO-H₂O₂-番红O化学发光体系。

Silver nanoparticles enhanced a novel TCPO-H₂O₂-safranin O chemiluminescence system for determination of 6-mercaptopurine.

作者信息

Biparva Pourya, Abedirad Seyed Mohammad, Kazemi Sayed Yahya

机构信息

Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Iran.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2015 Jun 15;145:454-460. doi: 10.1016/j.saa.2015.03.019. Epub 2015 Mar 10.

DOI:10.1016/j.saa.2015.03.019

PMID:25796015

Abstract

The present study deals with first attempt to introduce safranin O as the fluorophore for peroxyoxalate chemiluminescence system. The reaction of bis-(2,4,6-trichlorophenyl) oxalate (TCPO) with H2O2 catalyzed by silver nanoparticles can transfer energy to safranin O via the formation of dioxetanedione intermediate and emits orange-red light. The relationship between CL intensity and the concentration of TCPO, fluorophore, hydrogen peroxide and nanocatalyst was investigated. The Ag nanoparticles were synthesized by chemical reduction method and characterized using scanning electron microscopy, particle size analyzer and UV-spectroscopy. Moreover, the system was applied successfully to detect a drug, 6-mercaptopurine (6-MP) in pharmaceuticals. Under optimum conditions, a linear working range for 6-MP concentrations from 5.5 × 10(-7) to 5.5 × 10(-5)mol L(-1) (r>0.9831, n=6) was obtained with a detection limit of 1.6 × 10(-7)mol L(-1). The relative standard deviation for 6 repetitive determinations was less than 3.8% and recoveries of 98% and 103% were obtained.

摘要

本研究首次尝试引入番红O作为过氧草酸酯化学发光体系的荧光团。双（2,4,6-三氯苯基）草酸酯（TCPO）与由银纳米颗粒催化的过氧化氢反应，可通过形成二氧杂环丁二酮中间体将能量转移给番红O，并发出橙红色光。研究了化学发光强度与TCPO、荧光团、过氧化氢和纳米催化剂浓度之间的关系。通过化学还原法合成了银纳米颗粒，并使用扫描电子显微镜、粒度分析仪和紫外光谱对其进行了表征。此外，该体系成功应用于检测药物中的6-巯基嘌呤（6-MP）。在最佳条件下，6-MP浓度在5.5×10⁻⁷至5.5×10⁻⁵mol L⁻¹范围内呈线性工作范围（r>0.9831，n = 6），检测限为1.6×10⁻⁷mol L⁻¹。6次重复测定的相对标准偏差小于3.8%，回收率为98%和103%。