使用 TCPO-H2O2 化学发光测定银纳米粒子存在下的双嘧达莫。

Determination of dipyridamole using TCPO-H2O2 chemiluminescence in the presence of silver nanoparticles.

机构信息

School of Medicine, Xi'an Jiaotong University, Xi'an, People's Republic of China.

出版信息

Luminescence. 2011 Nov-Dec;26(6):703-9. doi: 10.1002/bio.1301. Epub 2011 Apr 19.

Abstract

The method is based on the fact that dipyridamole can enhance the chemiluminescence (CL) emission from the redox reaction of bis (2,4,6-tricholorophenyl) oxalate (TCPO) with H(2)O(2) in the presence of silver nanoparticles (AgNPs). The CL reaction mechanism was discussed. The effect of concentrations of TCPO, H(2)O(2), AgNPs and pH value on the CL reaction were investigated. Under the optimum conditions, the linear dynamic range was 1.0-1000 × 10(-9) g/mL and the detection limit (3σ) was 9 × 10(-10) g/mL. The relative standard deviation (RSD) was 4.8% for 1.0 × 10(-9) g/mL dipyridamole (n = 7). The proposed method has been successfully applied to the determination of dipyridamole tablets and the recovery was 99-103%.

摘要

该方法基于以下事实

双嘧达莫可以增强双（2,4,6-三氯苯基）草酸酯（TCPO）与过氧化氢（H₂O₂）在银纳米粒子（AgNPs）存在下的氧化还原反应的化学发光（CL）发射。讨论了 CL 反应机制。研究了 TCPO、H₂O₂、AgNPs 和 pH 值的浓度对 CL 反应的影响。在最佳条件下，线性动态范围为 1.0-1000×10⁻⁹ g/mL，检测限（3σ）为 9×10⁻¹⁰ g/mL。对于 1.0×10⁻⁹ g/mL 双嘧达莫（n=7），相对标准偏差（RSD）为 4.8%。该方法已成功应用于测定双嘧达莫片剂，回收率为 99-103%。