Yang Libin, Sun Xiaohui, Geng Xiaolin
Department of Orthopedics, the First Affiliated Hospital, Xinxiang Medical College, Xinxiang, China.
Pak J Pharm Sci. 2015 Mar;28(2 Suppl):667-70.
Discuss the internal mechanism of delaying degeneration of lumber intervertebral disc. The cartilage of lumbar intervertebral disc of SD rats was selected in vitro, then cultured by tissue explant method, and identified by HE staining, toluidine blue staining and immunofluorescence. The optimal concentration of psoralen was screened by cell proliferation assay and RT-PCR method. The cells in third generation with good growth situation is selected and placed in 6-well plate at concentration of 1×10(5)/well and its expression was tested. Compared to concentration of 0, the mRNA expression of Col2al (Collagen Ⅱ) secreted by was up regulated chondrocyte of lumbar intervertebral disc at the concentration of 12.5 and 25μM (P<0.0 or P<0.01). The aggrecan mRNA of psoralen group was higher than blank control group (P<0.01); compared with IL-1β induced group, the mRNA expression of Col2al was significantly increased but the mRNA expression of ADAMTS-5 was significantly decreased in psoralen group (P<0.01). These findings suggest that, psoralen can remit the degeneration of lumbar intervertebral disc induced by IL-1β to some extent, and affect the related factors of IL-1β signaling pathway.
探讨腰椎间盘退变延缓的内在机制。选取SD大鼠腰椎间盘软骨进行体外培养，采用组织块培养法培养，并用HE染色、甲苯胺蓝染色及免疫荧光进行鉴定。通过细胞增殖实验和RT-PCR法筛选补骨脂素的最佳浓度。选取生长状况良好的第三代细胞，以1×10(5)/孔的浓度接种于6孔板中并检测其表达。与0浓度相比，在12.5和25μM浓度下，腰椎间盘软骨细胞分泌的Col2al（Ⅱ型胶原蛋白）的mRNA表达上调（P<0.0或P<0.01）。补骨脂素组的聚集蛋白聚糖mRNA高于空白对照组（P<0.01）；与IL-1β诱导组相比，补骨脂素组Col2al的mRNA表达显著增加，而ADAMTS-5的mRNA表达显著降低（P<0.01）。这些结果表明，补骨脂素可在一定程度上缓解IL-1β诱导的腰椎间盘退变，并影响IL-1β信号通路的相关因子。
