长时间直立姿势会导致大鼠腰椎间盘发生退行性变化。
Prolonged upright posture induces degenerative changes in intervertebral discs in rat lumbar spine.
作者信息
Liang Qian-Qian, Zhou Quan, Zhang Min, Hou Wei, Cui Xue-Jun, Li Chen-Guang, Li Tian-Fang, Shi Qi, Wang Yong-Jun
机构信息
Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai, People's Republic of China.
出版信息
Spine (Phila Pa 1976). 2008 Sep 1;33(19):2052-8. doi: 10.1097/BRS.0b013e318183f949.
STUDY DESIGN
Both forelimbs of rats were amputated, and these rats were kept in the custom-made cages that kept the rats in prolonged upright posture. Pathologic changes were observed in the lumbar spine at different time points after the surgery.
OBJECTIVE
To investigate the effect of upright posture on intervertebral discs of rat lumbar spine.
SUMMARY OF BACKGROUND DATA
Previous studies have shown that increased axial forces on the spine can decrease the height of the intervertebral disc, but there are no data to indicate whether or not long-term and repeated assumption of the upright posture could result in degenerative changes.
METHODS
The forelimbs of 30 rats were amputated when they were 1-month old. These rats were kept in the custom-made cages and were forced to stand upright on their hind-limbs and tails to obtain water and food. Normal rats of the same ages kept in regular cages were used as control. The rats were killed at 5, 7, and 9 months after the surgery, and the intervertebral discs samples of lumbar spine were harvested for histologic and immunohistochemical studies. Total RNA isolated from these samples was used for real-time PCR of type II collagen (Col2alpha1), type X collagen (Col10alpha1), matrix metalloproteinase-13 (MMP-13), aggrecan, and disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS-5). RESULTS.: Histologic analysis showed degenerative changes of the intervertebral discs after surgery such as disordered collagen structure of endplate cartilage, fragmentation of annulus fibrosus, and decreased height of disc. Immunostaining revealed decreased protein level of type II collagen and increased protein expression of type X collagen. Real-time PCR showed upregulated expression of MMP 13, ADAMTS-5, and Col10alpha1 mRNA and downregulated mRNA expression of Col2alpha1 and aggrecan.
CONCLUSION
Long-term and repeated assumption of the upright stance accelerates disc degeneration in rats.
研究设计
将大鼠的双侧前肢截肢，并将这些大鼠置于特制的笼子中，使其保持长时间直立姿势。在手术后的不同时间点观察腰椎的病理变化。
目的
研究直立姿势对大鼠腰椎椎间盘的影响。
背景资料总结
先前的研究表明，脊柱上轴向力的增加会降低椎间盘的高度，但尚无数据表明长期反复保持直立姿势是否会导致退变改变。
方法
30只1月龄大鼠的前肢被截肢。将这些大鼠置于特制的笼子中，迫使它们用后肢和尾巴直立以获取水和食物。将同年龄的正常大鼠饲养在常规笼子中作为对照。在手术后5、7和9个月处死大鼠，采集腰椎椎间盘样本进行组织学和免疫组织化学研究。从这些样本中分离出的总RNA用于实时PCR检测II型胶原（Col2alpha1）、X型胶原（Col10alpha1）、基质金属蛋白酶-13（MMP-13）、聚集蛋白聚糖以及含血小板反应蛋白基序的解聚素和金属蛋白酶5（ADAMTS-5）。结果：组织学分析显示手术后椎间盘出现退变改变，如终板软骨胶原结构紊乱、纤维环破裂和椎间盘高度降低。免疫染色显示II型胶原蛋白水平降低，X型胶原蛋白表达增加。实时PCR显示MMP 13、ADAMTS-5和Col10alpha1 mRNA表达上调，Col2alpha1和聚集蛋白聚糖mRNA表达下调。
结论
长期反复保持直立姿势会加速大鼠椎间盘退变。
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