Fonseca-Alves Carlos Eduardo, Kobayashi Priscila Emiko, Rivera-Calderón Luis Gabriel, Laufer-Amorim Renée
Department of Veterinary Clinic, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista - UNESP, Botucatu 18618-970, São Paulo, Brazil.
Department of Veterinary Clinic, School of Veterinary Medicine and Animal Science, Univ. Estadual Paulista - UNESP, Botucatu 18618-970, São Paulo, Brazil.
Res Vet Sci. 2015 Jun;100:176-81. doi: 10.1016/j.rvsc.2015.03.001. Epub 2015 Mar 9.
The epithelial-mesenchymal transition (EMT) is a fundamental event responsible for the invasiveness and metastasis of epithelial tumours. The EMT has been described in many human cancers, but there are few reports of this phenomenon in veterinary oncology. Due to the importance of this process, the current study evaluated mesenchymal and epithelial marker protein expression in prostate lesions from dogs. Our results indicate both a loss of E-cadherin and translocation of β-catenin from the membrane to the cytoplasm and nucleus in the tumour group. Vimentin expression in the tumour group was higher than in normal tissue. All of the metastases were positive for prostate-specific antigen, pan-cytokeratin and E-cadherin, although fewer positive cells were present than in the primary tumours. The immunohistochemical results showed a loss of epithelial markers and a gain of a mesenchymal marker among metastatic cells, suggesting that the EMT occurs during the metastatic process of canine prostate carcinoma.
上皮-间质转化（EMT）是上皮性肿瘤侵袭和转移的一个基本过程。EMT在许多人类癌症中已有报道，但在兽医肿瘤学中关于这一现象的报道较少。鉴于这一过程的重要性，本研究评估了犬前列腺病变中间质和上皮标志物蛋白的表达。我们的结果表明，肿瘤组中E-钙黏蛋白缺失，β-连环蛋白从细胞膜转移至细胞质和细胞核。肿瘤组波形蛋白的表达高于正常组织。所有转移灶中前列腺特异性抗原、全细胞角蛋白和E-钙黏蛋白均呈阳性，尽管阳性细胞数量比原发性肿瘤少。免疫组化结果显示转移细胞中上皮标志物缺失，间质标志物增加，提示EMT发生在犬前列腺癌的转移过程中。
