Wan Ying-chun, Ma Hui-ting, Lu Bin
Institute of Environmental Medicine, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Rd, Wuhan, China.
Adv Biochem Eng Biotechnol. 2015;150:131-66. doi: 10.1007/10_2015_317.
When organic solvent-compatible molecularly imprinted polymers (MIPs) are used in aqueous environment, how to reduce nonspecific binding is a major challenge. By modifying the binding solvents and introducing appropriate washing and elution steps, even relatively hydrophobic MIPs can gain optimal rebinding selectivity in aqueous conditions. Furthermore, water-compatible MIPs that can be used to treat aqueous samples directly have been prepared. The use of hydrophilic co-monomers, the controlled surface modification through controlled radical polymerization, and the new interfacial molecular imprinting methods are different strategies to prepare water-compatible MIPs. By combining MIPs with other techniques, both organic solvent-compatible and water-compatible MIPs can display better functional performances in aqueous conditions. Intensive studies on MIPs in aqueous conditions can provide new MIPs with much-improved compatibilities that will lead to more interesting applications in biomedicine and biotechnology.
当将与有机溶剂兼容的分子印迹聚合物（MIPs）用于水性环境时，如何减少非特异性结合是一项重大挑战。通过改变结合溶剂并引入适当的洗涤和洗脱步骤，即使是相对疏水的MIPs也能在水性条件下获得最佳的再结合选择性。此外，还制备了可直接用于处理水性样品的与水兼容的MIPs。使用亲水性共聚单体、通过可控自由基聚合进行可控表面改性以及新的界面分子印迹方法是制备与水兼容的MIPs的不同策略。通过将MIPs与其他技术相结合，与有机溶剂兼容和与水兼容的MIPs在水性条件下都能展现出更好的功能性能。对水性条件下MIPs的深入研究可以提供兼容性大大提高的新型MIPs，这将在生物医学和生物技术领域带来更有趣的应用。
