肽 O⋯HN 氢键的特征：H 检测的 N/O 固态 NMR 光谱学。
Characterization of peptide O⋯HN hydrogen bonds H-detected N/O solid-state NMR spectroscopy.
机构信息
National High Magnetic Field Laboratory, 1800 East Paul Dirac Drive, Tallahassee, Florida, 32310, USA.
Department of Chemistry and Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA.
出版信息
Chem Commun (Camb). 2023 Mar 9;59(21):3111-3113. doi: 10.1039/d2cc07004a.
Abstract
High sensitivity and resolution solid-state NMR methods are reported, that straightforwardly select hydrogen-bonded N-O pairs from amongst all other nitrogen and oxygen sites in peptides, to aid protein secondary and tertiary structure determination. Significantly improved sensitivity is obtained with indirect H detection under fast MAS and stronger relayed dipole couplings.
摘要
报道了高灵敏度和分辨率的固态 NMR 方法，该方法可以直接从肽中的所有其他氮和氧位点中选择氢键结合的 N-O 对，以辅助蛋白质二级和三级结构的测定。在快速 MAS 和更强的接力偶极耦合下，通过间接 H 检测可获得显著提高的灵敏度。
相似文献
1
Characterization of peptide O⋯HN hydrogen bonds H-detected N/O solid-state NMR spectroscopy.肽 O⋯HN 氢键的特征：H 检测的 N/O 固态 NMR 光谱学。
Chem Commun (Camb). 2023 Mar 9;59(21):3111-3113. doi: 10.1039/d2cc07004a.
2
The (17)O-NMR shielding range and shielding time scale and detection of discrete hydrogen-bonded conformational states in peptides.肽中（17）O-NMR屏蔽范围、屏蔽时间尺度及离散氢键构象状态的检测
Biopolymers. 2001 Sep;59(3):125-30. doi: 10.1002/1097-0282(200109)59:3<125::AID-BIP1012>3.0.CO;2-A.
3
Determining the geometry of hydrogen bonds in solids with picometer accuracy by quantum-chemical calculations and NMR spectroscopy.通过量子化学计算和核磁共振光谱法以皮米精度确定固体中氢键的几何结构。
Chemphyschem. 2005 Feb;6(2):315-27. doi: 10.1002/cphc.200400338.
4
Proton-Based Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy.基于质子的超快魔角旋转固态核磁共振波谱学。
Acc Chem Res. 2017 Apr 18;50(4):1105-1113. doi: 10.1021/acs.accounts.7b00082. Epub 2017 Mar 29.
5
Fast-pulsing NMR techniques for the detection of weak interactions: successful natural abundance probe of hydrogen bonds in peptides.快速脉冲 NMR 技术用于检测弱相互作用：成功探测到肽中氢键的天然丰度探针。
Org Biomol Chem. 2013 Nov 21;11(43):7611-5. doi: 10.1039/c3ob41876f.
6
3hJ coupling between C(alpha) and H(N) across hydrogen bonds in proteins.蛋白质中通过氢键实现的C(α)与H(N)之间的3hJ耦合。
J Magn Reson. 2000 Apr;143(2):431-4. doi: 10.1006/jmre.2000.2048.
7
Probing hydrogen bonding and ion-carbonyl interactions by solid-state 17O NMR spectroscopy: G-ribbon and G-quartet.通过固态17O核磁共振光谱探究氢键和离子-羰基相互作用：G-带和G-四重奏。
J Am Chem Soc. 2007 Feb 28;129(8):2398-407. doi: 10.1021/ja067991m. Epub 2007 Feb 2.
8
A high-resolution 15N solid-state NMR study of collagen and related polypeptides. The effect of hydration on formation of interchain hydrogen bonds as the primary source of stability of the collagen-type triple helix.胶原蛋白及相关多肽的高分辨率¹⁵N 固态核磁共振研究。水合作用对链间氢键形成的影响，链间氢键是胶原型三螺旋稳定性的主要来源。
Eur J Biochem. 1994 Sep 1;224(2):729-34. doi: 10.1111/j.1432-1033.1994.00729.x.
9
Resolution and measurement of heteronuclear dipolar couplings of a noncrystalline protein immobilized in a biological supramolecular assembly by proton-detected MAS solid-state NMR spectroscopy.通过质子检测 MAS 固态 NMR 光谱法测定固定在生物超分子组装体中非晶态蛋白质的异核偶极耦合的分辨率和测量。
J Magn Reson. 2013 Dec;237:164-168. doi: 10.1016/j.jmr.2013.10.009. Epub 2013 Oct 26.
10
NMR studies of solid pentachlorophenol-4-methylpyridine complexes exhibiting strong OHN hydrogen bonds: geometric H/D isotope effects and hydrogen bond coupling cause isotopic polymorphism.NMR 研究表明，具有强 OHN 氢键的固体五氯苯酚-4-甲基吡啶配合物：几何 H/D 同位素效应和氢键耦合导致同位素多态性。
J Phys Chem A. 2012 Nov 26;116(46):11370-87. doi: 10.1021/jp305863n. Epub 2012 Aug 23.
引用本文的文献
1
O Solid-State NMR Spectroscopy of Lipid Membranes.脂质体的固态核磁共振波谱学
J Phys Chem B. 2024 Apr 18;128(15):3527-3537. doi: 10.1021/acs.jpcb.4c01016. Epub 2024 Apr 3.
2
Exploring Sulfur Sites in Proteins via Triple-Resonance H-Detected Se NMR.通过三重共振氢检测硒核磁共振探索蛋白质中的硫位点。
J Am Chem Soc. 2023 Oct 31;145(45):24648-56. doi: 10.1021/jacs.3c07225.
本文引用的文献
1
Residue-Specific High-Resolution O Solid-State Nuclear Magnetic Resonance of Peptides: Multidimensional Indirect H Detection and Magic-Angle Spinning.肽的残留特异性高分辨率 O 固体核磁共振：多维间接 H 检测和魔角旋转。
J Phys Chem Lett. 2022 Jul 21;13(28):6549-6558. doi: 10.1021/acs.jpclett.2c01777. Epub 2022 Jul 13.
2
Racing toward Fast and Effective O Isotopic Labeling and Nuclear Magnetic Resonance Spectroscopy of N-Formyl-MLF-OH and Associated Building Blocks.快速有效 O 同位素标记和 N-甲酰基-MLF-OH 及相关构建块的核磁共振波谱学研究进展。
J Phys Chem B. 2021 Nov 4;125(43):11916-11926. doi: 10.1021/acs.jpcb.1c07397. Epub 2021 Oct 25.
3
Functional stability of water wire-carbonyl interactions in an ion channel.水-羰基相互作用在离子通道中的功能稳定性。
Proc Natl Acad Sci U S A. 2020 Jun 2;117(22):11908-11915. doi: 10.1073/pnas.2001083117. Epub 2020 May 15.
4
O NMR studies of organic and biological molecules in aqueous solution and in the solid state.核磁共振在水溶液和固态中有机及生物分子的研究。
Prog Nucl Magn Reson Spectrosc. 2019 Oct-Dec;114-115:135-191. doi: 10.1016/j.pnmrs.2019.06.002. Epub 2019 Jun 13.
5
O MAS NMR Correlation Spectroscopy at High Magnetic Fields.高场 MAS NMR 相关波谱学。
J Am Chem Soc. 2017 Dec 13;139(49):17953-17963. doi: 10.1021/jacs.7b08989. Epub 2017 Nov 30.
6
Unleashing the Potential of O NMR Spectroscopy Using Mechanochemistry.利用机械化学释放 O NMR 光谱学的潜力。
Angew Chem Int Ed Engl. 2017 Jun 6;56(24):6803-6807. doi: 10.1002/anie.201702251. Epub 2017 Apr 28.
7
Amyloid Hydrogen Bonding Polymorphism Evaluated by (15)N{(17)O}REAPDOR Solid-State NMR and Ultra-High Resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.通过(15)N{(17)O}REAPDOR 固态核磁共振和超高分辨率傅里叶变换离子回旋共振质谱法评估淀粉样蛋白氢键多态性
Biochemistry. 2016 Apr 12;55(14):2065-8. doi: 10.1021/acs.biochem.5b01095. Epub 2016 Apr 1.
8
Solid-State ¹⁷O NMR studies of organic and biological molecules: Recent advances and future directions.有机和生物分子的固态¹⁷O核磁共振研究：最新进展与未来方向
Solid State Nucl Magn Reson. 2016 Feb;73:1-14. doi: 10.1016/j.ssnmr.2015.11.001. Epub 2015 Nov 30.
9
Protein resonance assignment at MAS frequencies approaching 100 kHz: a quantitative comparison of J-coupling and dipolar-coupling-based transfer methods.接近100kHz的魔角旋转频率下蛋白质的共振归属：基于J耦合和偶极耦合转移方法的定量比较
J Biomol NMR. 2015 Oct;63(2):165-86. doi: 10.1007/s10858-015-9975-y. Epub 2015 Aug 13.
10
Spinning proteins, the faster, the better?旋转蛋白质，转得越快越好？
J Magn Reson. 2015 Apr;253:71-9. doi: 10.1016/j.jmr.2015.01.012.
Zotero 插件