Park Jihyun, Park Jeongho, Jeong Jinju, Cho Kyung-Hyun, Choi Inho, Kim Jihoe
School of Biotechnology, Yeungnam University, Gyeongsan 712-749, South Korea.
School of Biotechnology, Yeungnam University, Gyeongsan 712-749, South Korea.
Biochem Biophys Res Commun. 2015 May 8;460(3):583-8. doi: 10.1016/j.bbrc.2015.03.073. Epub 2015 Mar 20.
Fetal bovine serum (FBS) is an essential culture supplement for adipocyte differentiation of various adipogenic precursor cells. Adipocyte differentiation greatly varies depending on the type of serum in the differentiation medium. In this study, we found that FBS supported adipocyte differentiation of 3T3-L1 cells to a significantly higher extent than other types of bovine serum such as adult bovine serum (ABS). This differential adipogenic effect of bovine serum was shown to be due to the protein contents of bovine sera, indicating the presence of an adipogenic protein(s) in FBS. Serum proteome analysis identified tetranectin as an adipogenic protein. The adipogenic effect of tetranectin was confirmed by supplementation of FBS-containing differentiation medium with anti-tetranectin antibody, which suppressed adipocyte differentiation of 3T3-L1 cells. These results demonstrate that tetranectin is an adipogenic serum protein mediating the adipogenic effect of FBS.
胎牛血清（FBS）是各种脂肪生成前体细胞脂肪分化必不可少的培养补充剂。脂肪细胞分化在很大程度上取决于分化培养基中血清的类型。在本研究中，我们发现FBS比其他类型的牛血清（如成年牛血清（ABS））更能显著促进3T3-L1细胞的脂肪分化。牛血清的这种差异脂肪生成作用被证明是由于牛血清的蛋白质含量，这表明FBS中存在一种脂肪生成蛋白。血清蛋白质组分析确定纤连蛋白为一种脂肪生成蛋白。通过向含FBS的分化培养基中添加抗纤连蛋白抗体来证实纤连蛋白的脂肪生成作用，该抗体抑制了3T3-L1细胞的脂肪分化。这些结果表明，纤连蛋白是一种介导FBS脂肪生成作用的脂肪生成血清蛋白。
