Ali Muhammad Yousuf, Pavasovic Ana, Amin Shorash, Mather Peter B, Prentis Peter J
School of Earth, Environmental and Biological Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia.
School of Biomedical Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane Qld, 4001, Australia.
Mar Genomics. 2015 Aug;22:11-3. doi: 10.1016/j.margen.2015.03.004. Epub 2015 Mar 19.
We undertook deep sequencing of gill transcriptomes from two freshwater crayfish, Cherax cainii and Cherax destructor, in order to generate genomic resources for future genomics research. Over 83 and 100 million high quality (quality score (Q)≥30) paired-end Illumina reads (150 bp) were assembled into 147,101 and 136,622 contigs in C. cainii and C. destructor, respectively. A total of 24,630 and 23,623 contigs received significant BLASTx hits and allowed the identification of multiple gill expressed candidate genes associated with pH and salinity balance. These functionally annotated transcripts will provide a resource to facilitate comparative genomic research in the genus Cherax, and in particular allow insights into respiratory and osmoregulatory physiology of this group of animals.
为了生成用于未来基因组学研究的基因组资源，我们对两种淡水小龙虾——凯恩氏螯虾（Cherax cainii）和破坏螯虾（Cherax destructor）的鳃转录组进行了深度测序。超过8300万和1亿高质量（质量得分（Q）≥30）的双端Illumina读段（150碱基对）分别在凯恩氏螯虾和破坏螯虾中组装成147,101和136,622个重叠群。共有24,630和23,623个重叠群获得了显著的BLASTx匹配结果，并鉴定出多个与pH值和盐度平衡相关的鳃表达候选基因。这些功能注释的转录本将为促进螯虾属的比较基因组研究提供资源，特别是有助于深入了解这组动物的呼吸和渗透调节生理学。
