Rong J U, Wen Zeng, Rong W U, Zhichun Feng
Department of Neonatology, Chengdu Women's and Children's Central Hospital, Chongqing Medical University, Chengdu, Sichuan 610091, P.R. China.
Neonatal Medical Center, Huaian Maternity and Child Healthcare Hospital Affiliated to Yangzhou University Medical Academy, Huaian, Jiangsu 223002, P.R. China.
Biomed Rep. 2015 Mar;3(2):242-246. doi: 10.3892/br.2014.405. Epub 2014 Dec 17.
Due to their capacity to self-replicate or produce specific differentiated cell types, neural stem cells (NSCs) and bone marrow derived-mesenchymal stem cells (BMSCs) are potential sources for cell transplantation therapies, particularly for neural injury. However, the interaction between NSCs and BMSCs during differentiation has not yet been defined. The interaction is believed to improve the effectiveness and efficiency of cell therapy. In the present study, human NSCs and BMSCs were cultured and the Transwell co-culture system was used to observe the interplay between NSCs and BMSCs during differentiation. The results revealed that NSCs promoted BMSCs to differentiate into neurons and NSCs; whereas, BMSCs did not affect the differentiation of NSCs. Simultaneously, co-culture increased the concentration of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), which are secreted by NSCs and BMSCs. The present findings suggest that co-culture of NSCs and BMSCs can promote the differentiation and this process may be modulated by BDNF and NGF.
由于神经干细胞（NSCs）和骨髓间充质干细胞（BMSCs）具有自我复制或产生特定分化细胞类型的能力，它们是细胞移植治疗的潜在来源，尤其是对于神经损伤。然而，NSCs和BMSCs在分化过程中的相互作用尚未明确。据信这种相互作用会提高细胞治疗的有效性和效率。在本研究中，培养了人NSCs和BMSCs，并使用Transwell共培养系统观察NSCs和BMSCs在分化过程中的相互作用。结果显示，NSCs促进BMSCs分化为神经元和NSCs；而BMSCs不影响NSCs的分化。同时，共培养增加了NSCs和BMSCs分泌的脑源性神经营养因子（BDNF）和神经生长因子（NGF）的浓度。本研究结果表明，NSCs和BMSCs的共培养可促进分化，且这一过程可能受BDNF和NGF的调节。
