体外光动力疗法低辐照剂量阈值的特征分析
Characterizing low fluence thresholds for in vitro photodynamic therapy.
作者信息
Hartl Brad A, Hirschberg Henry, Marcu Laura, Cherry Simon R
机构信息
Department of Biomedical Engineering, University of California, Davis, Davis, CA 95616, USA.
Beckman Laser Institute and Medical Clinic, University of California, Irvine, Irvine, CA 92697, USA.
出版信息
Biomed Opt Express. 2015 Feb 10;6(3):770-9. doi: 10.1364/BOE.6.000770. eCollection 2015 Mar 1.
Abstract
The translation of photodynamic therapy (PDT) to the clinic has mostly been limited to superficial diseases where traditional light delivery is noninvasive. To overcome this limitation, a variety of mechanisms have been suggested to noninvasively deliver light to deep tissues. This work explores the minimum amount of light required by these methods to produce a meaningful PDT effect in the in vitro setting under representative low fluence and wavelength conditions. This threshold was found to be around 192 mJ/cm(2) using the clinically approved photosensitizer aminolevulinic acid and 12 mJ/cm(2) for the more efficient, second generation photosensitizer TPPS2a.
摘要
光动力疗法（PDT）向临床的转化大多局限于传统光传输为非侵入性的浅表疾病。为克服这一局限性，人们提出了多种将光非侵入性传输至深部组织的机制。这项研究探讨了在具有代表性的低能量密度和波长条件下，这些方法在体外环境中产生有意义的PDT效应所需的最小光量。使用临床批准的光敏剂氨基乙酰丙酸时，该阈值约为192 mJ/cm²，而对于效率更高的第二代光敏剂TPPS2a，该阈值为12 mJ/cm²。
相似文献
1
Characterizing low fluence thresholds for in vitro photodynamic therapy.体外光动力疗法低辐照剂量阈值的特征分析
Biomed Opt Express. 2015 Feb 10;6(3):770-9. doi: 10.1364/BOE.6.000770. eCollection 2015 Mar 1.
2
Activating Photodynamic Therapy in vitro with Cerenkov Radiation Generated from Yttrium-90.利用钇-90产生的切伦科夫辐射进行体外光动力激活疗法。
J Environ Pathol Toxicol Oncol. 2016;35(2):185-92. doi: 10.1615/JEnvironPatholToxicolOncol.2016016903.
3
Low-fluence rate, long duration photodynamic therapy in glioma mouse model using organic light emitting diode (OLED).使用有机发光二极管（OLED）对胶质瘤小鼠模型进行低光通量率、长时间光动力疗法。
Photodiagnosis Photodyn Ther. 2015 Sep;12(3):504-10. doi: 10.1016/j.pdpdt.2015.04.007. Epub 2015 Apr 29.
4
Development of a novel indwelling balloon applicator for optimizing light delivery in photodynamic therapy.一种用于优化光动力疗法中光传输的新型留置球囊施加器的研发。
Lasers Surg Med. 2001;29(5):406-12. doi: 10.1002/lsm.10005.
5
Measurement of Cyanine Dye Photobleaching in Photosensitizer Cyanine Dye Conjugates Could Help in Optimizing Light Dosimetry for Improved Photodynamic Therapy of Cancer.测量光敏剂花菁染料缀合物中的花菁染料光漂白情况有助于优化光疗剂量，从而提高癌症的光动力疗法效果。
Molecules. 2018 Jul 24;23(8):1842. doi: 10.3390/molecules23081842.
6
Interstitial 5-ALA photodynamic therapy and glioblastoma: Preclinical model development and preliminary results.间质5-氨基乙酰丙酸光动力疗法与胶质母细胞瘤：临床前模型的建立及初步结果
Photodiagnosis Photodyn Ther. 2016 Mar;13:218-224. doi: 10.1016/j.pdpdt.2015.07.169. Epub 2015 Jul 23.
7
Assessing daylight & low-dose rate photodynamic therapy efficacy, using biomarkers of photophysical, biochemical and biological damage metrics in situ.评估日光和低剂量率光动力疗法的疗效，使用光物理、生化和生物损伤指标的生物标志物进行原位评估。
Photodiagnosis Photodyn Ther. 2017 Dec;20:227-233. doi: 10.1016/j.pdpdt.2017.10.005. Epub 2017 Oct 14.
8
Topical photodynamic therapy at low fluence rates--theory and practice.低能量密度的局部光动力疗法——理论与实践
J Photochem Photobiol B. 2001 Apr;60(1):37-43. doi: 10.1016/s1011-1344(01)00116-6.
9
Photodynamic therapy of actinic keratosis at varying fluence rates: assessment of photobleaching, pain and primary clinical outcome.不同光通量率下光动力疗法治疗光化性角化病：光漂白、疼痛及主要临床结果评估
Br J Dermatol. 2004 Dec;151(6):1204-12. doi: 10.1111/j.1365-2133.2004.06211.x.
10
How to avoid local side effects of bladder photodynamic therapy: impact of the fluence rate.如何避免膀胱光动力疗法的局部副作用：光辐照度的影响。
J Urol. 2013 Aug;190(2):731-6. doi: 10.1016/j.juro.2013.01.046. Epub 2013 Jan 23.
引用本文的文献
1
Photodynamic Therapy in Primary Cutaneous Skin Lymphoma-Systematic Review.光动力疗法治疗原发性皮肤淋巴瘤——系统评价
J Clin Med. 2025 Apr 24;14(9):2956. doi: 10.3390/jcm14092956.
2
Recent development of nanomaterials-based PDT to improve immunogenic cell death.基于纳米材料的 PDT 最近的发展以改善免疫原性细胞死亡。
Photochem Photobiol Sci. 2024 Oct;23(10):1983-1998. doi: 10.1007/s43630-024-00638-y. Epub 2024 Sep 25.
3
In Vitro Antimicrobial Photodynamic Therapy for () and (MRSA) Inhibition Using a Green Light Source.使用绿色光源的体外抗菌光动力疗法对（）和耐甲氧西林金黄色葡萄球菌（MRSA）的抑制作用
Pharmaceutics. 2024 Apr 9;16(4):518. doi: 10.3390/pharmaceutics16040518.
4
Reactive Oxygen Species Produced by 5-Aminolevulinic Acid Photodynamic Therapy in the Treatment of Cancer.5-氨基酮戊酸光动力疗法治疗癌症产生的活性氧物种。
Int J Mol Sci. 2023 May 18;24(10):8964. doi: 10.3390/ijms24108964.
5
Combination of Two Photosensitisers in Anticancer, Antimicrobial and Upconversion Photodynamic Therapy.两种光敏剂在抗癌、抗菌及上转换光动力治疗中的联合应用
Pharmaceuticals (Basel). 2023 Apr 19;16(4):613. doi: 10.3390/ph16040613.
6
A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source.一种受生物矿化启发的合成胶体长效磷光体作为多色活体光源的方法。
Sci Adv. 2022 Jul 29;8(30):eabo6743. doi: 10.1126/sciadv.abo6743.
7
Photodynamic Therapy Review: Principles, Photosensitizers, Applications, and Future Directions.光动力疗法综述：原理、光敏剂、应用及未来方向。
Pharmaceutics. 2021 Aug 25;13(9):1332. doi: 10.3390/pharmaceutics13091332.
8
A Warp-Knitted Light-Emitting Fabric-Based Device for In Vitro Photodynamic Therapy: Description, Characterization, and Application on Human Cancer Cell Lines.一种用于体外光动力疗法的基于经编发光织物的装置：描述、表征及其在人癌细胞系上的应用。
Cancers (Basel). 2021 Aug 15;13(16):4109. doi: 10.3390/cancers13164109.
9
Systematic Review and Meta-Analysis of In Vitro Anti-Human Cancer Experiments Investigating the Use of 5-Aminolevulinic Acid (5-ALA) for Photodynamic Therapy.关于使用5-氨基乙酰丙酸（5-ALA）进行光动力治疗的体外抗人类癌症实验的系统评价和荟萃分析。
Pharmaceuticals (Basel). 2021 Mar 7;14(3):229. doi: 10.3390/ph14030229.
10
Metronomic photodynamic therapy using an implantable LED device and orally administered 5-aminolevulinic acid.经皮给药的光动力疗法：使用可植入 LED 装置和口服 5-氨基酮戊酸。
Sci Rep. 2020 Dec 16;10(1):22017. doi: 10.1038/s41598-020-79067-7.
本文引用的文献
1
A light blanket for intraoperative photodynamic therapy.一种用于术中光动力治疗的轻质毯子。
Proc SPIE Int Soc Opt Eng. 2013 Jul 13;7380:73801W. doi: 10.1117/12.823064.
2
X-ray-induced nanoparticle-based photodynamic therapy of cancer.基于纳米颗粒的X射线诱导癌症光动力疗法
Nanomedicine (Lond). 2014 Oct;9(15):2339-51. doi: 10.2217/nnm.13.198. Epub 2014 Jan 28.
3
Efficacy of 5-aminolevulinic acid-mediated photodynamic therapy using light-emitting diodes in human colon cancer cells.5-氨基酮戊酸介导的发光二极管光动力疗法对人结肠癌细胞的疗效。
Oncol Rep. 2013 Mar;29(3):911-6. doi: 10.3892/or.2013.2220. Epub 2013 Jan 3.
4
In vivo photodynamic therapy using upconversion nanoparticles as remote-controlled nanotransducers.体内上转换纳米颗粒光动力治疗：作为远程控制的纳米换能器。
Nat Med. 2012 Oct;18(10):1580-5. doi: 10.1038/nm.2933. Epub 2012 Sep 16.
5
Photodynamic inactivation of mammalian viruses and bacteriophages.光动力灭活哺乳动物病毒和噬菌体。
Viruses. 2012 Jul;4(7):1034-74. doi: 10.3390/v4071034. Epub 2012 Jun 26.
6
Photodynamic antifungal chemotherapy.光动力抗真菌化学疗法。
Photochem Photobiol. 2012 May-Jun;88(3):512-22. doi: 10.1111/j.1751-1097.2012.01107.x. Epub 2012 Mar 1.
7
Two-photon induced luminescence, singlet oxygen generation, cellular uptake and photocytotoxic properties of amphiphilic Ru(II) polypyridyl-porphyrin conjugates as potential bifunctional photodynamic therapeutic agents.两亲性 Ru(II) 多吡啶-卟啉配合物的双光子诱导发光、单线态氧生成、细胞摄取和光细胞毒性作为潜在的双功能光动力治疗剂。
Org Biomol Chem. 2011 Sep 7;9(17):6004-10. doi: 10.1039/c1ob05415e. Epub 2011 Jul 12.
8
Upconverting nanoparticles.上转换纳米粒子。
Angew Chem Int Ed Engl. 2011 Jun 20;50(26):5808-29. doi: 10.1002/anie.201005159. Epub 2011 May 30.
9
Photodynamic therapy of cancer: an update.光动力疗法治疗癌症：最新进展。
CA Cancer J Clin. 2011 Jul-Aug;61(4):250-81. doi: 10.3322/caac.20114. Epub 2011 May 26.
10
Photodynamic therapy in dermatology: state-of-the-art.皮肤科光动力疗法：最新进展。
Photodermatol Photoimmunol Photomed. 2010 Jun;26(3):118-32. doi: 10.1111/j.1600-0781.2010.00507.x.
Zotero 插件