An Ran, Wang Chunmin, Turek John, Machaty Zoltan, Nolte David D
Dept. of Physics, Purdue University, West Lafayette IN 47907 USA.
Dept. of Animal Sciences, Purdue University, West Lafayette IN 47907 USA.
Biomed Opt Express. 2015 Feb 25;6(3):963-76. doi: 10.1364/BOE.6.000963. eCollection 2015 Mar 1.
The success of assisted reproductive technologies relies on accurate assessment of reproductive viability at successive stages of development for oocytes and embryos. The current scoring system used to select good-quality oocytes relies on morphologically observable traits and hence is indirect and subjective. Biodynamic imaging may provide an objective approach to oocyte and embryo assessment by measuring physiologically-relevant dynamics. Biodynamic imaging is a coherence-gated approach to 3D tissue imaging that uses digital holography to perform low-coherence speckle interferometry to capture dynamic light scattering from intracellular motions. The changes in intracellular activity during cumulus oocyte complex maturation, before and after in vitro fertilization, and the subsequent development of the zygote and blastocyst provide a new approach to the assessment of preimplant candidates.
辅助生殖技术的成功依赖于在卵母细胞和胚胎发育的连续阶段对生殖活力进行准确评估。当前用于选择优质卵母细胞的评分系统依赖于形态学上可观察到的特征，因此是间接且主观的。生物动力学成像可以通过测量生理相关动力学为卵母细胞和胚胎评估提供一种客观方法。生物动力学成像是一种用于三维组织成像的相干门控方法，它利用数字全息术进行低相干散斑干涉测量，以捕获细胞内运动产生的动态光散射。卵丘卵母细胞复合体成熟过程中、体外受精前后细胞内活性的变化，以及随后受精卵和囊胚的发育，为评估植入前候选对象提供了一种新方法。
