Fayon Michael, Andrieux Annick, Bara Imane, Rebola Muriel, Labbé André, Marthan Roger, Berger Patrick
Université de Bordeaux, Centre de Recherche Cardio-thoracique de Bordeaux, U1045, F-33000, Bordeaux, France; CHU de Bordeaux, Hôpital Pellegrin-Enfants, Pneumologie Pédiatrique, Centre d'Investigation Clinique (CIC 1401), F-33076, Bordeaux, France.
Université de Bordeaux, Centre de Recherche Cardio-thoracique de Bordeaux, U1045, F-33000, Bordeaux, France.
PLoS One. 2015 Mar 23;10(3):e0122446. doi: 10.1371/journal.pone.0122446. eCollection 2015.
We compared the proliferation of neonatal and adult airway smooth muscle cells (ASMC) with no/moderate lung disease, in glucose- (energy production by glycolysis) or glucose-free medium (ATP production from mitochondrial oxidative phosphorylations only), in response to 10% fetal calf serum (FCS) and PDGF-AA. In the presence of glucose, cell counts were significantly greater in neonatal vs. adult ASMC. Similarly, neonatal ASMC DNA synthesis in 10% FCS and PDGF-AA, and [Ca2+]i responses in the presence of histamine were significantly enhanced vs. adults. In glucose-free medium, cell proliferation was preserved in neonatal cells, unlike in adult cells, with concomitant increased porin (an indicator of mitochondrial activity) protein expression. Compared to adults, stimulated neonatal human ASMC are in a rapid and robust proliferative phase and have the capacity to respond disproportionately under abnormal environmental conditions, through increased mitochondrial biogenesis and altered calcium homeostasis.
我们比较了无/轻度肺部疾病的新生儿和成人气道平滑肌细胞（ASMC）在含葡萄糖培养基（通过糖酵解产生能量）或无葡萄糖培养基（仅通过线粒体氧化磷酸化产生ATP）中，对10%胎牛血清（FCS）和血小板衍生生长因子AA（PDGF-AA）的增殖反应。在有葡萄糖存在的情况下，新生儿ASMC的细胞计数显著高于成人ASMC。同样，与成人相比，在10% FCS和PDGF-AA存在下，新生儿ASMC的DNA合成以及在组胺存在下的[Ca2+]i反应显著增强。在无葡萄糖培养基中，新生儿细胞的细胞增殖得以保留，而成人细胞则不然，同时孔蛋白（线粒体活性指标）的蛋白表达增加。与成人相比，受刺激的新生儿人ASMC处于快速且强劲的增殖阶段，并且有能力在异常环境条件下通过增加线粒体生物合成和改变钙稳态做出不成比例的反应。
