CHU Besançon, Service d'Hygiène Hospitalière, Boulevard Fleming, Besançon, 25030 France.
CHU Besançon, Service d'Hygiène Hospitalière, Boulevard Fleming, Besançon, 25030 France.
J Hosp Infect. 2015 Jul;90(3):208-12. doi: 10.1016/j.jhin.2014.12.022. Epub 2015 Feb 28.
Sequence type 131 (ST131) is a predominant lineage among extraintestinal pathogenic Escherichia coli. It plays a major role in the worldwide dissemination of extended-spectrum β-lactamase (ESBL)-producing E. coli. The ST131 pandemic is mainly the result of clonal expansion of the single well-adapted subclone H30-Rx, which is acquired in hospitals more frequently than other ESBL-producing E. coli clones.
To develop a rapid method using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to identify ST131 for infection control purposes.
Peak biomarkers of ST131 were identified from the mass spectrum profiles of 109 E. coli isolates (including 50 ST131 isolates).
The models accurately identified ST131 isolates from mass spectrum profiles obtained with and without protein extraction.
The rapid identification of ST131 isolates with MALDI-TOF MS can be easily implemented in the laboratory, and could help to target infection control measures in patients carrying multi-drug-resistant E. coli that are more likely to spread.
序列类型 131（ST131）是肠外致病性大肠杆菌中的主要谱系。它在世界范围内传播产生扩展谱β-内酰胺酶（ESBL）的大肠杆菌中起着重要作用。ST131 大流行主要是由于单一适应性良好的亚克隆 H30-Rx 的克隆扩张所致，与其他产生 ESBL 的大肠杆菌克隆相比，该亚克隆在医院中更频繁地获得。
开发一种使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）的快速方法，用于感染控制目的识别 ST131。
从 109 株大肠杆菌分离株（包括 50 株 ST131 分离株）的质谱谱图中鉴定出 ST131 的峰生物标志物。
该模型可准确识别未经和经蛋白提取后从质谱图谱中获得的 ST131 分离株。
MALDI-TOF MS 快速鉴定 ST131 分离株可在实验室中轻松实施，并有助于针对携带更有可能传播的多药耐药大肠杆菌的患者实施感染控制措施。
