使用VITEK MS Plus基质辅助激光解吸电离飞行时间质谱法检测产超广谱β-内酰胺酶大肠埃希菌中的序列类型131克隆群
Detection of Escherichia coli sequence type 131 clonal group among extended-spectrum β-lactamase-producing E. coli using VITEK MS Plus matrix-assisted laser desorption ionization-time of flight mass spectrometry.
作者信息
Matsumura Yasufumi, Yamamoto Masaki, Nagao Miki, Tanaka Michio, Takakura Shunji, Ichiyama Satoshi
机构信息
Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin-kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
出版信息
J Microbiol Methods. 2015 Dec;119:7-9. doi: 10.1016/j.mimet.2015.09.016. Epub 2015 Sep 28.
PMID:26415529
Abstract
We investigated the performance of the VITEK MS Plus system for the detection of Escherichia coli sequence type 131 (ST131) among extended-spectrum β-lactamase-producing E. coli isolates. The SARAMIS software could discriminate the 67 ST131 isolates from 82 non-ST131 isolates with a sensitivity of 86.6% and a specificity of 95.1%.
摘要
我们研究了VITEK MS Plus系统在产超广谱β-内酰胺酶的大肠杆菌分离株中检测大肠杆菌序列类型131（ST131）的性能。SARAMIS软件能够从82株非ST131分离株中鉴别出67株ST131分离株，灵敏度为86.6%，特异性为95.1%。
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