Zhang Min, Xiao Gongnian, Thring Ronald W, Chen Wan, Zhou Huabin, Yang Hailong
College of Life and Environmental Science, Wenzhou University, Chashan University Town, Wenzhou, 325035, China.
Appl Biochem Biotechnol. 2015 May;176(1):253-66. doi: 10.1007/s12010-015-1571-9. Epub 2015 Mar 24.
Natural melanin is of great potential value and application in the fields of pharmacology, cosmetics, and functional foods. In the present study, statistically designed experiments were conducted for the optimization of the media to enhance the production of melanin by submerged culture of Auricularia auricula. Glucose, tyrosine, peptone, and CaCO3 were found to have significant effects (P < 0.015) on melanin biosynthesis by a Plackett-Burman experimental design and subsequently optimized using response surface methodology. Optimal media were obtained at the following concentrations: glucose, 0.90 g/L; tyrosine, 6.68 g/L; peptone, 6.99 g/L; and CaCO3, 6.75 g/L. The validity of the optimum media was verified in separate experiments in which the melanin yield of 1008.08 mg/L was obtained under optimum conditions, compared with 306.52 mg/L at other conditions, i.e., a 3.29-fold increase. Furthermore, the important physical and chemical properties of A. auricula melanin were determined. The findings from the present study indicate that large-scale production of natural melanin by submerged culture of A. auricular could be a useful approach.
天然黑色素在药理学、化妆品和功能性食品领域具有巨大的潜在价值和应用前景。在本研究中，通过统计学设计的实验对培养基进行优化，以提高黑木耳深层培养黑色素的产量。通过Plackett-Burman实验设计发现，葡萄糖、酪氨酸、蛋白胨和碳酸钙对黑色素生物合成有显著影响（P < 0.015），随后使用响应面法进行优化。获得的最佳培养基浓度如下：葡萄糖0.90 g/L；酪氨酸6.68 g/L；蛋白胨6.99 g/L；碳酸钙6.75 g/L。在单独的实验中验证了最佳培养基的有效性，在最佳条件下黑色素产量达到1008.08 mg/L，而在其他条件下为306.52 mg/L，即提高了3.29倍。此外，还测定了黑木耳黑色素的重要物理和化学性质。本研究结果表明，通过黑木耳深层培养大规模生产天然黑色素可能是一种有用的方法。
