Alsharafi Walid, Xiao Bo
Department of Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
CNS Neurol Disord Drug Targets. 2015;14(8):1096-102. doi: 10.2174/1871527314666150317225945.
Recently, microRNAs (miRNAs) are emerging as new regulators in the pathogenesis of temporal lobe epilepsy (TLE) and playing a major role in the inflammatory and immune processes. The aim of the present study was to evaluate the dynamic expression of brain-specific miR-183 and miR-135a, brain-enriched miR-125b and miR-128 and inflammation-related miR-30c and miR-27a. Status epilepticus evoked by pilocarpine administeration was used to induce epilepsy in rats. Quantitative polymerase chain reaction was performed on rat hippocampus 2 hours, 3 weeks and 2 months following pilocarpine-induced status epilepticus, representing the acute, latent, and chronic phases, respectively. Expression levels were also measured in hippocampus obtained from TLE patients and normal controls. In the rat model, miR-183, miR-135a and miR-125b were detected upregulated during the acute and chronic phases compared to controls, but not during the latent phase. miR-30c and miR-27a were upregulated in the acute and chronic phases of TLE, while in the latent phase miR-30c was downregulated and miR-27a was upregulated. On the other hand, miR-128 showed significantly downregulated in all phases of TLE development. In TLE patients, miR-183, miR- 135a, miR-125b, miR-30c and miR-27a were upregulated, whereas miR-128 was downregulated. Our study revealed upregulation of miR-183, miR-135a and miR-125b in the seizure-related phases and TLE patients, suggesting that all may provide a potential therapeutic approach for the treatment of TLE, whereas the dysregulation of miR-128, miR-30c and miR-27a may suggest different functions during the process of TLE development.
最近，微小RNA（miRNA）作为颞叶癫痫（TLE）发病机制中的新型调节因子崭露头角，并在炎症和免疫过程中发挥重要作用。本研究旨在评估脑特异性miR-183和miR-135a、脑富集miR-125b和miR-128以及炎症相关miR-30c和miR-27a的动态表达。通过给予匹鲁卡品诱发癫痫持续状态来诱导大鼠癫痫。在匹鲁卡品诱发癫痫持续状态后的2小时、3周和2个月对大鼠海马进行定量聚合酶链反应，分别代表急性期、潜伏期和慢性期。还对来自TLE患者和正常对照的海马进行了表达水平检测。在大鼠模型中，与对照组相比，miR-183、miR-135a和miR-125b在急性期和慢性期被检测到上调，但在潜伏期未上调。miR-30c和miR-27a在TLE的急性期和慢性期上调，而在潜伏期miR-30c下调，miR-27a上调。另一方面，miR-128在TLE发展的所有阶段均显著下调。在TLE患者中，miR-183、miR-135a、miR-125b、miR-30c和miR-27a上调，而miR-128下调。我们的研究揭示了miR-183、miR-135a和miR-125b在癫痫相关阶段和TLE患者中的上调，表明它们都可能为TLE的治疗提供潜在的治疗方法，而miR-128、miR-30c和miR-27a的失调可能表明在TLE发展过程中具有不同的功能。
