溶血磷脂酰丝氨酸与佛波酯十四酰佛波醇乙酸酯在大鼠肥大细胞中的协同作用。

Synergism between lysophosphatidylserine and the phorbol ester tetradecanoylphorbolacetate in rat mast cells.

作者信息

Battistella A, Mietto L, Toffano G, Palatini P, Bigon E, Bruni A

出版信息

Life Sci. 1985 Apr 22;36(16):1581-7. doi: 10.1016/0024-3205(85)90383-2.

Abstract

In rat peritoneal mast cells tetradecanoylphorbolacetate (TPA) induced a non cytotoxic histamine release in the absence of extracellular calcium. The addition of calcium prevented the TPA effect but micromolar concentrations of lysophosphatidylserine (lysoPS) converted the calcium-induced inhibition into a stimulation. Other lysophospholipids were inactive. In agreement with a mutual influence between lysoPS and TPA, minimal TPA concentrations enhanced the calcium-dependent histamine release induced by lysoPS in the presence of nerve-growth factor. It is proposed that the calcium-dependent pathway promoted by lysoPS and the activation of protein kinase C by TPA act synergically to induce histamine release from mast cells.

摘要

在大鼠腹膜肥大细胞中，十四酰佛波醇乙酸酯（TPA）在无细胞外钙的情况下诱导了非细胞毒性的组胺释放。添加钙可阻止TPA的作用，但微摩尔浓度的溶血磷脂酰丝氨酸（lysoPS）可将钙诱导的抑制转化为刺激。其他溶血磷脂则无活性。与lysoPS和TPA之间的相互影响一致，在存在神经生长因子的情况下，最低TPA浓度增强了lysoPS诱导的钙依赖性组胺释放。有人提出，lysoPS促进的钙依赖性途径和TPA对蛋白激酶C的激活协同作用，诱导肥大细胞释放组胺。