Fawwaz R A, Oluwole S, Wang T S, Kuromoto N, Iga C, Hardy M A, Alderson P O
Radiology. 1985 May;155(2):483-6. doi: 10.1148/radiology.155.2.2580333.
Factors that might affect the biodistribution and clinical utility of radiolabeled lymphocytes were evaluated in experimental animals. Indium-111 (In-111) labeled lymphocytes (10(7)-10(9) syngeneic or allogeneic cells; 1-10 microCi [.037-.37 MBq]/10(8) cells) obtained from peripheral blood, lymph node, or spleen were found in significant amounts in the lymphoid tissues of Lewis rats as early as 3 hours after infusion. A progressive increase in nodal activity with concomitant fall of activity in other organs followed, indicating active recirculation of the lymphocytes. However, In-111 labeled thymocytes or xenogeneic lymphocytes failed to accumulate in lymphoid tissue. In vitro irradiation of the In-111 labeled lymphocytes (100-400 rads [1-4 Gy]) before in vivo administration and increase of the In-111 to 40 microCi [1.48 MBq]/10(8) lymphocytes resulted in no detectable lymphocyte recirculation and/or reduced localization in lymphoid tissue. Splenectomized animals and those sensitized to an organ allograft before cell infusion showed increased activity in their bone marrow. These results suggest that the source of the injected cells, cell irradiation dose level, and host sensitization should be considered when radiolabeled lymphocytes are being prepared for use in clinical diagnosis and therapy.
在实验动物中评估了可能影响放射性标记淋巴细胞生物分布和临床应用的因素。从外周血、淋巴结或脾脏获取的铟 - 111（In - 111）标记的淋巴细胞（10⁷ - 10⁹ 同基因或异基因细胞；1 - 10微居里[.037 -.37兆贝可]/10⁸ 细胞），早在输注后3小时就在Lewis大鼠的淋巴组织中大量发现。随后淋巴结活性逐渐增加，同时其他器官的活性下降，表明淋巴细胞有活跃的再循环。然而，In - 111标记的胸腺细胞或异种淋巴细胞未能在淋巴组织中积聚。在体内给药前对In - 111标记的淋巴细胞进行体外照射（100 - 400拉德[1 - 4戈瑞]），并将In - 111增加到40微居里[1.48兆贝可]/10⁸ 淋巴细胞，导致无法检测到淋巴细胞再循环和/或在淋巴组织中的定位减少。脾切除的动物以及在细胞输注前对器官同种异体移植致敏的动物，其骨髓活性增加。这些结果表明，在准备将放射性标记淋巴细胞用于临床诊断和治疗时，应考虑注射细胞的来源、细胞照射剂量水平和宿主致敏情况。
