Department of Chemistry and Center for Self-Assembled Chemical Structures (CSACS-CRMAA), McGill University, 801 Sherbrooke Street West, Montreal, Quebec H3A 0B8, Canada.
Nat Chem. 2015 Apr;7(4):295-300. doi: 10.1038/nchem.2184. Epub 2015 Feb 23.
DNA nanotubes offer a high aspect ratio and rigidity, attractive attributes for the controlled assembly of hierarchically complex linear arrays. It is highly desirable to control the positioning of rungs along the backbone of the nanotubes, minimize the polydispersity in their manufacture and reduce the building costs. We report here a solid-phase synthesis methodology in which, through a cyclic scheme starting from a 'foundation rung' specifically bound to the surface, distinct rungs can be incorporated in a predetermined manner. Each rung is orthogonally addressable. Using fluorescently tagged rungs, single-molecule fluorescence studies demonstrated the robustness and structural fidelity of the constructs and confirmed the incorporation of the rungs in quantitative yield (>95%) at each step of the cycle. Prototype structures that consisted of up to 20 repeat units, about 450 nm in contour length, were constructed. Combined, the solid-phase synthesis strategy described and its visualization through single-molecule spectroscopy show good promise for the production of custom-made DNA nanotubes.
DNA 纳米管具有高纵横比和刚性，这是用于控制组装层次复杂线性阵列的有吸引力的属性。控制梯级在纳米管主链上的定位、最大限度地减少其制造过程中的多分散性并降低构建成本是非常可取的。我们在这里报告了一种固相合成方法，通过从特定结合到表面的“基础梯级”开始的循环方案，可以以预定的方式并入不同的梯级。每个梯级都可以正交寻址。使用荧光标记的梯级，单分子荧光研究证明了构建体的稳健性和结构保真度，并确认在循环的每一步都以定量产率（>95%）掺入梯级。构建了由多达 20 个重复单元组成的约 450nm 轮廓长度的原型结构。综上所述，通过单分子光谱学进行的固相合成策略描述及其可视化显示了生产定制 DNA 纳米管的良好前景。
