Alexander Stefanie-Ann, Kyi Caroline, Schiesser Carl H
ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, Australia.
Org Biomol Chem. 2015 Apr 28;13(16):4751-9. doi: 10.1039/c5ob00284b.
A series of 23 nitroxides () was tested for biofilm modulatory activity using a crystal violet staining technique. 3-(Dodecane-1-thiyl)-4-(hydroxymethyl)-2,2,5,5-tetramethyl-1-pyrrolinoxyl () was found to significantly suppress biofilm formation and elicit dispersal events in both Pseudomonas aeruginosa and mixed-culture biofilms. Twitching and swarming motilities were enhanced by nitroxide , leaving the planktonic-specific swimming motility unaffected and suggesting that the mechanism of -mediated biofilm modulation is linked to the hyperactivation of surface-associated cell motilities. Preliminary structure-activity relationship studies identify the dodecanethiyl chain, hydroxymethyl substituent and the free radical moiety to be structural features pertinent to the anti-biofilm activity of .
使用结晶紫染色技术对一系列23种氮氧化物（）进行了生物膜调节活性测试。发现3-（十二烷-1-硫基）-4-（羟甲基）-2,2,5,5-四甲基-1-吡咯啉-N-氧基（）能显著抑制铜绿假单胞菌和混合培养生物膜中的生物膜形成并引发分散事件。氮氧化物增强了颤动和群体运动能力，而浮游特异性游泳运动能力不受影响，这表明介导生物膜调节的机制与表面相关细胞运动的过度激活有关。初步的构效关系研究确定十二烷硫基链、羟甲基取代基和自由基部分是与的抗生物膜活性相关的结构特征。
