Zhu Xiangjia, Zhang Keke, He Wenwen, Yang Jin, Sun Xinghuai, Jiang Chunhui, Dai Jinhui, Lu Yi
Department of Ophthalmology, Eye and Ear, Nose, and Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, China.
Department of Ophthalmology, Eye and Ear, Nose, and Throat Hospital, Fudan University, 83 Fenyang Road, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, China.
Exp Eye Res. 2016 Jan;142:13-8. doi: 10.1016/j.exer.2015.03.017. Epub 2015 Mar 22.
High myopia has long been recognized as an inflammation-related disease, and high myopic eyes are thought to have a proinflammatory internal microenvironment, which might predispose to the occurrence of certain inflammation-related complications such as fibrotic capsular contraction syndrome after cataract surgery. Therefore, the purpose of this study was to detect inflammatory cytokines expressed in the aqueous humor (AH) of high myopic cataract (HMC) patients. The cytokines were screened using a RayBio Human Cytokine Antibody Array in AH samples from 15 age-related cataract (ARC) patients and 15 HMC patients. Those detected by the screening assays were verified using a Bio-Plex Suspension Array System in AH samples from 35 ARC patients and 45 HMC patients. The cytokine antibody array showed that the expression level of interleukin-1 receptor antagonist (IL-1ra) in the AH was higher in ARC than in HMC, whereas opposite trends were found for monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T-cell expressed and presumably secreted (RANTES), IL-8, platelet-derived growth factor-BB, and IL-6 (all P < 0.05). In the verification assay using the suspension cytokine array, only the expression levels of IL-1ra and MCP-1 were significantly different between the ARC and HMC groups (P = 0.014 and 0.038, respectively); these results were confirmed by western blot assays. Our results demonstrated that the expression of IL-1ra was significantly lower and the expression of MCP-1 was significantly higher in the AH of HMC than in ARC, suggestive of a proinflammatory status in the anterior chamber of HMC eyes.
高度近视长期以来一直被认为是一种与炎症相关的疾病，高度近视眼被认为具有促炎的内部微环境，这可能易患某些与炎症相关的并发症，如白内障手术后的纤维化囊膜收缩综合征。因此，本研究的目的是检测高度近视性白内障（HMC）患者房水中表达的炎性细胞因子。使用RayBio人细胞因子抗体阵列对15例年龄相关性白内障（ARC）患者和15例HMC患者的房水样本中的细胞因子进行筛选。通过筛选测定法检测到的细胞因子，在35例ARC患者和45例HMC患者的房水样本中使用Bio-Plex悬浮阵列系统进行验证。细胞因子抗体阵列显示，ARC患者房水中白细胞介素-1受体拮抗剂（IL-1ra）的表达水平高于HMC患者，而单核细胞趋化蛋白-1（MCP-1）、活化调节正常T细胞表达和可能分泌因子（RANTES）、IL-8、血小板衍生生长因子-BB和IL-6则呈现相反趋势（均P<0.05）。在使用悬浮细胞因子阵列的验证试验中，ARC组和HMC组之间只有IL-1ra和MCP-1的表达水平有显著差异（分别为P=0.014和0.038）；这些结果通过蛋白质免疫印迹法得到证实。我们的结果表明，HMC患者房水中IL-1ra的表达显著低于ARC患者，而MCP-1的表达显著高于ARC患者，提示HMC眼的前房处于促炎状态。
