Department of Ophthalmology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing, China.
Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University; Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing, China.
Mol Vis. 2020 Mar 5;26:150-157. eCollection 2020.
To analyze the expression of 440 human cytokines in aqueous humor of high myopic patients with cataracts.
Eighty-five patients with cataracts were recruited in this study. In the screening stage, the RayBio G-Series Human Cytokine Antibody Array 440 was used to assay the aqueous humor samples collected from nine high myopic patients with cataracts and eight non-myopic patients with cataracts right before the surgery. The array was further used for verification of the screened cytokines, with aqueous humor samples obtained from 34 eyes of high myopic patients with cataracts and 34 eyes of non-myopic patients with cataracts.
Compared with the non-myopic patients with cataracts, the expression levels of decorin, receptor activator of NF-kB (RANK), angiopoietin-1 (ANG-1), C-X-C motif ligand 16 (CXCL16), β-inducible gene-h3 (bIG-H3), insulin-like growth factor-binding protein 2 (IGFBP-2), and interleukin-17B (IL-17B) were statistically significantly higher in high myopic patients with cataracts (all p<0.000114). The matrix metalloproteinase-2 (MMP-2) level also increased in the aqueous humor of high myopic patients with cataracts (p = 0.0034). The concentrations of ANG-1 and MMP-2 were also increased in the aqueous humor of the confirmatory stage (all p<0.05).
In this study, numerous cytokines in aqueous humor were detected in high myopic patients with cataracts and non-myopic patients with cataracts, and it was confirmed that the MMP-2 level in the aqueous humor of patients with high myopia was statistically significantly increased. Further verification also revealed the elevation of ANG-1 in the aqueous humor of high myopic patients with cataracts, which suggests that ANG-1 may be related to the pathogenesis of high myopia.
分析白内障高度近视患者房水中 440 个人类细胞因子的表达。
本研究纳入了 85 例白内障患者。在筛选阶段，使用 RayBio G 系列人类细胞因子抗体阵列 440 检测了 9 例白内障高度近视患者和 8 例非近视白内障患者手术前采集的房水样本。该阵列进一步用于筛选细胞因子的验证，使用 34 例白内障高度近视患者和 34 例非近视白内障患者的房水样本。
与非近视白内障患者相比，白内障高度近视患者房水中的核心蛋白聚糖、核因子 κB 受体激活剂（RANK）、血管生成素-1（ANG-1）、C-X-C 基序配体 16（CXCL16）、β-诱导基因-h3（bIG-H3）、胰岛素样生长因子结合蛋白 2（IGFBP-2）和白细胞介素-17B（IL-17B）的表达水平明显更高（均 p<0.000114）。房水中基质金属蛋白酶-2（MMP-2）水平也升高（p = 0.0034）。确认阶段房水中 ANG-1 和 MMP-2 的浓度也升高（均 p<0.05）。
在这项研究中，在白内障高度近视患者和非近视白内障患者的房水中检测到了多种细胞因子，并证实了高度近视患者房水中 MMP-2 水平显著升高。进一步验证还发现房水中 ANG-1 的升高，提示 ANG-1 可能与高度近视的发病机制有关。
