Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029 People's Republic of China.
Indian J Microbiol. 2015 Jun;55(2):131-9. doi: 10.1007/s12088-015-0513-0. Epub 2015 Jan 15.
3-Hydroxypropionic acid (3-HP) is a commercially valuable platform chemical from which an array of C3 compounds can be generated. Klebsiella pneumoniae has been considered a promising species for biological production of 3-HP. Despite a wealth of reports related to 3-HP biosynthesis in K. pneumoniae, its commercialization is still in infancy. The major hurdle hindering 3-HP overproduction lies in the poor understanding of glycerol dissimilation in K. pneumoniae. To surmount this problem, this review aims to portray a picture of 3-HP biosynthesis, involving 3-HP-synthesizing strains, biochemical attributes, metabolic pathways and key enzymes. Inspired by the state-of-the-art advances in metabolic engineering and synthetic biology, here we advocate protocols for overproducing 3-HP in K. pneumoniae. These protocols range from cofactor regeneration, alleviation of metabolite toxicity, genome editing, remodeling of transport system, to carbon flux partition via logic gate. The feasibility for these protocols was also discussed.
3-羟基丙酸（3-HP）是一种具有商业价值的平台化学品，可从中生成一系列 C3 化合物。肺炎克雷伯氏菌被认为是生物生产 3-HP 的有前途的物种。尽管有大量关于肺炎克雷伯氏菌中 3-HP 生物合成的报道，但它的商业化仍处于起步阶段。阻碍 3-HP 过度生产的主要障碍在于对肺炎克雷伯氏菌中甘油分解代谢的理解不足。为了克服这个问题，本综述旨在描绘 3-HP 生物合成的图景，包括 3-HP 合成菌株、生化特性、代谢途径和关键酶。受代谢工程和合成生物学最新进展的启发，我们在这里提倡在肺炎克雷伯氏菌中过量生产 3-HP 的方案。这些方案包括辅因子再生、缓解代谢物毒性、基因组编辑、运输系统重塑以及通过逻辑门进行碳通量分配。还讨论了这些方案的可行性。
