Woolf N J, Eckenstein F, Butcher L L
Neurosci Lett. 1983 Sep 30;40(2):93-8. doi: 10.1016/0304-3940(83)90285-9.
Cholinergic projections from the basal forebrain to some regions of the frontal cortex were studied by infusing propidium iodide (PI), a fluorescent tracer, into areas 6 and 10 and microscopically assessing the cellular co-localization of PI and immunohistochemically demonstrated choline-O-acetyltransferase (ChAT). The same brain sections were additionally processed for acetylcholinesterase (AChE, pharmacohistochemical regimen) and Nissl material (cresyl violet stain). Basal forebrain neurons projecting to the frontal cortex were found primarily in nucleus basalis, but others were located in association with the substantia innominata/lateral preoptic area, magnocellular preoptic area, and ansa lenticularis. These projection neurons were large (greater than 25 micrometers in maximum soma extent), demonstrated ChAT-like immunoreactivity, stained intensely for AChE following systemic administration of bis-(1-methylethyl)phosphorofluoridate, and were highly chromophilic.
通过将荧光示踪剂碘化丙啶（PI）注入6区和10区，并在显微镜下评估PI与免疫组织化学显示的胆碱-O-乙酰转移酶（ChAT）的细胞共定位，研究了从基底前脑到额叶皮质某些区域的胆碱能投射。对相同的脑切片额外进行乙酰胆碱酯酶（AChE，药物组织化学方法）和尼氏物质（甲酚紫染色）处理。发现投射到额叶皮质的基底前脑神经元主要位于基底核，但其他神经元则与无名质/外侧视前区、大细胞视前区和豆状襻相关。这些投射神经元体积较大（最大胞体直径大于25微米），显示出ChAT样免疫反应性，在全身给予双（1-甲基乙基）磷酰氟后对AChE染色强烈，且嗜色性高。
