不同哺乳动物和鸡血清中α1-微球蛋白的免疫学分析。α1-微球蛋白在灵长类动物中比其他动物大5 - 8千道尔顿。

Immunological analysis of alpha 1-microglobulin in different mammalian and chicken serum. alpha 1-Microglobulin is 5-8 kilodaltons larger in primates.

作者信息

Akerström B

出版信息

J Biol Chem. 1985 Apr 25;260(8):4839-44.

Abstract

Heterologous radioimmunoassays for a semiquantitative analysis of alpha 1-microglobulin were developed, exploiting the binding between polyclonal rabbit or goat antisera against human, guinea pig, or rat alpha 1-microglobulin and 125I-labeled human, guinea pig, or rat alpha 1-microglobulin. Homologues of this protein were detected in human, guinea pig, Rhesus monkey, rat, mouse, rabbit, goat, horse, and cow serum by inhibition of a set of heterologous radioimmunoassays. Serum proteins were separated by gel chromatography, and fractions were pooled, concentrated, and radiolabeled with 125I. By immunoprecipitation of the radioiodinated serum pools with heterologous anti-alpha 1-microglobulin-sera, and separating the precipitates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, analogues of alpha 1-microglobulin were isolated from serum of man, guinea pig, Rhesus monkey, rat, mouse, horse, and chicken. The apparent molecular weight of alpha 1-microglobulin was 31,000-32,000 in human and monkey serum and 24,000-26,000 in guinea pig, rat, mouse, horse, and chicken serum. The possibility of an addition of a 5,000-8,000-Da peptide in primate alpha 1-microglobulin is discussed.

摘要

开发了用于α1-微球蛋白半定量分析的异源放射免疫测定法，利用针对人、豚鼠或大鼠α1-微球蛋白的兔或山羊多克隆抗血清与125I标记的人、豚鼠或大鼠α1-微球蛋白之间的结合。通过一组异源放射免疫测定法的抑制作用，在人、豚鼠、恒河猴、大鼠、小鼠、兔、山羊、马和牛血清中检测到了该蛋白的同源物。血清蛋白通过凝胶色谱法分离，各组分合并、浓缩并用125I进行放射性标记。通过用异源抗α1-微球蛋白血清对放射性碘化血清池进行免疫沉淀，并通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离沉淀物，从人、豚鼠、恒河猴、大鼠、小鼠、马和鸡的血清中分离出α1-微球蛋白的类似物。α1-微球蛋白在人血清和猴血清中的表观分子量为31,000-32,000，在豚鼠、大鼠、小鼠、马和鸡血清中为24,000-26,000。讨论了在灵长类动物α1-微球蛋白中添加5,000-8,000道尔顿肽的可能性。