Ravid R, Swaab D F, Pool C W
J Endocrinol. 1985 Apr;105(1):133-40. doi: 10.1677/joe.0.1050133.
The rat kidney and brain are major target organs for vasopressin (VP). A procedure was developed for immunocytochemical staining of VP and its binding sites in the kidney. This procedure involved preincubation of kidney sections with the ligand, followed by immunocytochemical detection of VP. The staining in renal tubules from Wistar rats was enhanced by preincubation of tissue sections with increasing concentrations of VP (6-6000 nmol/l). Staining was present in the epithelium of distal convolutions and collecting ducts (medullary and cortical portions) and more pronounced in the apical zone of the tubular epithelium. With high concentrations of VP in the preincubation, staining was also obtained in the thick ascending limb of the loop of Henle. There was no staining under any circumstances in proximal tubules. In the kidney of the Brattleboro rat homozygous for hypothalamic diabetes insipidus (DI) which congenitally lacks VP but responds to the peptide, exactly the same staining pattern was observed after preincubation with VP, but the maximal staining was less intense. The VP binding to the DI rat kidney, after 2 weeks treatment with VP (using Accurel implants), reached levels seen in the Wistar kidney after in-vitro preincubation with high doses of VP.
大鼠的肾脏和大脑是抗利尿激素（VP）的主要靶器官。已开发出一种用于肾脏中VP及其结合位点免疫细胞化学染色的方法。该方法包括用配体对肾脏切片进行预孵育，然后对VP进行免疫细胞化学检测。用浓度递增的VP（6 - 6000 nmol/l）对Wistar大鼠的肾脏组织切片进行预孵育，可增强肾小管中的染色。染色出现在远曲小管和集合管（髓质和皮质部分）的上皮细胞中，在肾小管上皮细胞的顶端区域更为明显。预孵育时使用高浓度的VP，在髓袢升支粗段也可获得染色。在任何情况下，近端小管均无染色。遗传性缺乏VP但对该肽有反应的下丘脑性尿崩症（DI）纯合Brattleboro大鼠的肾脏，在用VP预孵育后观察到的染色模式完全相同，但最大染色强度较弱。用VP（使用Accurel植入物）处理2周后，DI大鼠肾脏中的VP结合达到了Wistar大鼠肾脏在体外经高剂量VP预孵育后的水平。
