Wissinger W L, Petters R M, Cervone T H, Mettus R V
Mutat Res. 1985 May;149(3):375-83. doi: 10.1016/0027-5107(85)90154-x.
Bleomycin (BM) induced dominant-lethal genetic lesions in the gametes of both sexes of adult Bracon hebetor wasps following ingestion. This effect was demonstrated by decreased fertility in the unfertilized eggs of treated females as well as in eggs fertilized by mature sperm derived from treated males. Death in the unhatched eggs occurred prior to blastoderm formation indicating BM caused chromosome breakage. Affected ovarian cells were limited to those exposed to BM between the developmental stages of vitellogenesis and early metaphase I. Sperm-associated fertility reductions were temporary in duration with substantial reductions being observed starting on the second day following the initiation of oviposition. BM lowered general egg-formation capacity in treated females but, unlike the fertility effects, decreased fecundity was caused by somatic debility. Although the BM-induced genetic damage observed in B. hebetor was radiomimetic in nature, the time of appearance and duration of the effects did not correspond with previous patterns found when B. hebetor adults were exposed to X-rays. While the differences could be due to the BM detoxification or the repair of BM-induced chromosome damage, further investigation would be required to demonstrate the presence of such systems in this hymemopteran.
博来霉素（BM）在成年杂拟谷盗黄蜂雌雄两性的配子中摄入后会诱导显性致死性遗传损伤。这种效应通过处理过的雌性未受精卵以及由处理过的雄性产生的成熟精子受精的卵的生育力下降得以证明。未孵化卵的死亡发生在胚盘形成之前，表明BM导致了染色体断裂。受影响的卵巢细胞仅限于在卵黄发生和减数第一次分裂前期早期发育阶段之间暴露于BM的细胞。与精子相关的生育力下降持续时间是暂时的，在产卵开始后的第二天开始观察到大幅下降。BM降低了处理过的雌性的总体产卵能力，但与生育力效应不同的是，繁殖力下降是由体细胞衰弱引起的。尽管在杂拟谷盗中观察到的BM诱导的遗传损伤本质上是放射模拟性的，但其出现时间和效应持续时间与杂拟谷盗成虫暴露于X射线时发现的先前模式不相符。虽然差异可能是由于BM解毒或BM诱导的染色体损伤修复，但需要进一步研究来证明这种膜翅目昆虫中存在此类系统。
