Kan C C, Solomon E, Belt K T, Chain A C, Hiorns L R, Fey G
Proc Natl Acad Sci U S A. 1985 Apr;82(8):2282-6. doi: 10.1073/pnas.82.8.2282.
Six alpha 2-macroglobulin (alpha 2M) cDNA clones were isolated from a human liver cDNA library by using synthetic oligonucleotides as hybridization probes. One of these, p alpha 2M1, carries a 4.6 kilobase-pair insert, which was sequenced. The insert contains the coding sequences for the mature alpha 2M polypeptide (1451 amino acids) and for a 23-amino acid signal peptide at the NH2 terminus of the precursor pro-alpha 2M. At the 3' end of the insert a poly(A) addition signal A-A-T-A-A-A and part of the poly(A) tail of the messenger RNA were found. The protein sequence deduced from the nucleotide sequence agrees with the published alpha 2M amino acid sequence for all except three residues. The alpha 2M locus was assigned to human chromosome 12 by Southern blot analysis with DNA from a panel of mouse/human somatic cell hybrids, using alpha 2M cDNA as a hybridization probe.
利用合成寡核苷酸作为杂交探针，从人肝脏cDNA文库中分离出6个α2-巨球蛋白（α2M）cDNA克隆。其中一个名为pα2M1，携带一个4.6千碱基对的插入片段，并对其进行了测序。该插入片段包含成熟α2M多肽（1451个氨基酸）的编码序列以及前体α2M前体NH2末端的一个23个氨基酸的信号肽。在插入片段的3'端发现了一个聚腺苷酸化信号A-A-T-A-A-A和信使RNA的部分聚腺苷酸尾巴。从核苷酸序列推导的蛋白质序列与已发表的α2M氨基酸序列除三个残基外完全一致。使用α2M cDNA作为杂交探针，通过对一组小鼠/人类体细胞杂种的DNA进行Southern印迹分析，将α2M基因座定位到人类12号染色体上。
