Ogedengbe Mosun E, Qvarnstrom Yvonne, da Silva Alexandre J, Arrowood Michael J, Barta John R
Department of Pathobiology, University of Guelph, Guelph, ON, Canada.
United States Centers for Disease Control and Prevention, Atlanta, GA, USA.
Int J Parasitol. 2015 May;45(6):361-5. doi: 10.1016/j.ijpara.2015.02.006. Epub 2015 Mar 23.
The near complete mitochondrial genome for Cyclospora cayetanensis is 6184 bp in length with three protein-coding genes (Cox1, Cox3, CytB) and numerous lsrDNA and ssrDNA fragments. Gene arrangements were conserved with other coccidia in the Eimeriidae, but the C. cayetanensis mitochondrial genome is not circular-mapping. Terminal transferase tailing and nested PCR completed the 5'-terminus of the genome starting with a 21 bp A/T-only region that forms a potential stem-loop. Regions homologous to the C. cayetanensis mitochondrial genome 5'-terminus are found in all eimeriid mitochondrial genomes available and suggest this may be the ancestral start of eimeriid mitochondrial genomes.
卡耶塔环孢子虫的近乎完整的线粒体基因组长度为6184 bp，包含三个蛋白质编码基因（Cox1、Cox3、CytB）以及众多的长链核糖体DNA和短链核糖体DNA片段。基因排列与艾美耳科的其他球虫一致，但卡耶塔环孢子虫的线粒体基因组并非环状图谱。末端转移酶加尾和巢式PCR完成了基因组5'端的测序，起始于一个仅由21 bp的A/T组成的区域，该区域形成一个潜在的茎环结构。在所有已有的艾美耳科线粒体基因组中均发现了与卡耶塔环孢子虫线粒体基因组5'端同源的区域，这表明这可能是艾美耳科线粒体基因组的祖先起始位点。
