Arabacı Taner, Kermen Eda, Özkanlar Seçkin, Köse Oğuz, Kara Adem, Kızıldağ Alper, Duman Şuayip Burak, Ibişoğlu Ebru
Department of Periodontology, Faculty of Dentistry, Atatürk University, Erzurum, Turkey.
Department of Biochemistry, Faculty of Veterinary Medicine, Atatürk University.
J Periodontol. 2015 Jul;86(7):874-81. doi: 10.1902/jop.2015.140599. Epub 2015 Mar 27.
The present study aims to investigate the effects of systemic melatonin administration on alveolar bone resorption in experimental periodontitis in rats.
Twenty-four male Sprague-Dawley rats were divided into three groups (control, experimental periodontitis [Ped], and experimental periodontitis treated with melatonin [Mel-Ped]). For periodontitis induction, first molars were ligatured submarginally for 4 weeks. After ligature removal, rats in the Mel-Ped group were treated with a daily single dose of 10 mg/kg body weight melatonin for 15 consecutive days. At the end of the study, intracardiac blood samples and mandible tissues were obtained for histologic, biochemical, and radiographic analysis. Serum markers related to bone turnover, calcium, phosphorus, bone alkaline phosphatase (b-ALP), and terminal C telopeptide of collagen Type I (CTX) were analyzed. Myeloperoxidase levels were determined in gingival tissue homogenates, and receptor activator of nuclear factor-kappa B ligand (RANKL) activation was analyzed in the mandible samples stereologically. Alveolar bone loss was also evaluated radiographically in the mandible samples of each group.
Melatonin treatment decreased serum CTX levels and increased b-ALP levels. Serum calcium and phosphorus levels were not statistically different among groups (P >0.05). Alveolar bone resorption and myeloperoxidase activity were statistically higher in the Ped group compared to the Mel-Ped group (P <0.05). Immunohistochemical staining of RANKL and osteoclast activity were significantly lower in the Mel-Ped group compared to the Ped group (P <0.05).
This study reveals that melatonin treatment significantly inhibits regional alveolar bone resorption and contributes to periodontal healing in an experimental periodontitis rat model.
本研究旨在探讨全身给予褪黑素对大鼠实验性牙周炎牙槽骨吸收的影响。
将24只雄性Sprague-Dawley大鼠分为三组（对照组、实验性牙周炎组[Ped]和褪黑素治疗实验性牙周炎组[Mel-Ped]）。为诱导牙周炎，将第一磨牙龈下结扎4周。结扎去除后，Mel-Ped组大鼠连续15天每日单剂量给予10 mg/kg体重的褪黑素。在研究结束时，采集心脏内血液样本和下颌骨组织进行组织学、生化和影像学分析。分析与骨转换、钙、磷、骨碱性磷酸酶（b-ALP）和I型胶原C末端肽（CTX）相关的血清标志物。测定牙龈组织匀浆中的髓过氧化物酶水平，并对下颌骨样本进行立体学分析以评估核因子κB受体活化因子配体（RANKL）的活化情况。还对每组下颌骨样本进行影像学评估牙槽骨吸收情况。
褪黑素治疗降低了血清CTX水平，提高了b-ALP水平。各组间血清钙和磷水平无统计学差异（P>0.05）。与Mel-Ped组相比，Ped组的牙槽骨吸收和髓过氧化物酶活性在统计学上更高（P<0.05）。与Ped组相比，Mel-Ped组RANKL的免疫组化染色和破骨细胞活性显著降低（P<0.05）。
本研究表明，褪黑素治疗可显著抑制局部牙槽骨吸收，并有助于实验性牙周炎大鼠模型的牙周愈合。
