Fiorentino T V, Hribal M L, Andreozzi F, Perticone M, Sciacqua A, Perticone F, Sesti G
Department of Medical and Surgical Sciences, Viale Europa, University Magna-Græcia of Catanzaro, 88100 Catanzaro, Italy.
Department of Medical and Surgical Sciences, Viale Europa, University Magna-Græcia of Catanzaro, 88100 Catanzaro, Italy.
Nutr Metab Cardiovasc Dis. 2015 May;25(5):510-7. doi: 10.1016/j.numecd.2015.02.007. Epub 2015 Feb 25.
To evaluate if complement C3 is associated with insulin secretion, as suggested by recent in vitro studies, independently of confounders including adiposity measures.
1010 nondiabetic subjects were stratified into quartiles according to complement C3 values. Insulin secretion was assessed using indexes derived from oral glucose tolerance test (OGTT) in the whole study group and from intravenous glucose tolerance test (IVGTT) in a subgroup (n = 110). Significant differences between quartiles of C3 were observed in body mass index (BMI), waist, fat mass, blood pressure, total cholesterol, high density lipoprotein (HDL), triglycerides, fasting and 2-h post-load glucose, fasting insulin, C reactive protein (hsCRP), fibrinogen, aspartate aminotransferase (AST), alanine aminotransferase (ALT), complement C4, and insulin sensitivity with C3 quartiles exhibiting graded increases in cardio-metabolic risk factors. Differences in insulin secretion indexes between C3 quartiles remained significant after adjustment for age, gender, BMI, insulin sensitivity, blood pressure, total cholesterol, HDL, triglycerides, hsCRP, fibrinogen, and complement C4 levels (P < 0.0001). A multivariable regression analysis revealed that complement C3 is a contributor of insulin secretion, explaining 2.4% and 1.9% of variation of the Stumvoll index for first-phase and second-phase insulin secretion, respectively, and 2.1% of variation of the InsAUC30/GluAUC30 index, independently of gender, age, BMI, waist, fat mass, blood pressure, total cholesterol, HDL, triglycerides, hsCRP, fibrinogen, AST, ALT.
Complement C3 concentrations are associated with insulin secretion independently of important determinants of glucose homeostasis such as gender, age, adiposity, subclinical inflammation, and insulin sensitivity.
正如近期体外研究所示，评估补体C3是否与胰岛素分泌相关，且独立于包括肥胖指标在内的混杂因素。
根据补体C3值将1010名非糖尿病受试者分为四分位数组。在整个研究组中，使用口服葡萄糖耐量试验（OGTT）得出的指标评估胰岛素分泌；在一个亚组（n = 110）中，使用静脉葡萄糖耐量试验（IVGTT）评估胰岛素分泌。在体重指数（BMI）、腰围、脂肪量、血压、总胆固醇、高密度脂蛋白（HDL）、甘油三酯、空腹及负荷后2小时血糖、空腹胰岛素、C反应蛋白（hsCRP）、纤维蛋白原、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、补体C4以及胰岛素敏感性方面，观察到C3四分位数组之间存在显著差异，C3四分位数组的心血管代谢危险因素呈梯度增加。在调整年龄、性别、BMI、胰岛素敏感性、血压、总胆固醇、HDL、甘油三酯、hsCRP、纤维蛋白原和补体C4水平后，C3四分位数组之间的胰岛素分泌指标差异仍然显著（P < 0.0001）。多变量回归分析显示，补体C3是胰岛素分泌的一个影响因素，分别解释了第一相和第二相胰岛素分泌的Stumvoll指数变异的2.4%和1.9%，以及InsAUC30/GluAUC30指数变异的2.1%，且独立于性别、年龄、BMI、腰围、脂肪量、血压、总胆固醇、HDL、甘油三酯、hsCRP、纤维蛋白原、AST、ALT。
补体C3浓度与胰岛素分泌相关，且独立于葡萄糖稳态的重要决定因素，如性别、年龄、肥胖、亚临床炎症和胰岛素敏感性。
