一种用于测定人血浆中环丙沙星的简单高效液相色谱 - 紫外检测法。

A simple HPLC-UV method for the determination of ciprofloxacin in human plasma.

作者信息

Vella Janis, Busuttil Francesca, Bartolo Nicolette Sammut, Sammut Carmel, Ferrito Victor, Serracino-Inglott Anthony, Azzopardi Lilian M, LaFerla Godfrey

机构信息

Department of Pharmacy, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2015 May 1;989:80-5. doi: 10.1016/j.jchromb.2015.01.006. Epub 2015 Jan 12.

DOI:10.1016/j.jchromb.2015.01.006

PMID:25813900

Abstract

A rapid and sensitive HPLC-UV method for the determination of ciprofloxacin in human plasma is described. Protein precipitation with acetonitrile was used to separate the drug from plasma protein. An ACE(®) 5 C18 column (250 mm×4.6 mm, 5 μm) with an isocratic mobile phase consisting of phosphate buffer (pH 2.7) and acetonitrile (77:23, v/v) was used for separation. The UV detector was set at 277 nm. The method was validated in the linear range of 0.05-8 μg/ml with acceptable inter- and intra-assay precision, accuracy and stability. The method is simple and rapid and can be used to quantify this widely used antibiotic in the plasma of patients suffering from Peripheral Arterial Disease.

摘要

本文描述了一种快速灵敏的HPLC-UV法测定人血浆中的环丙沙星。采用乙腈沉淀蛋白法将药物与血浆蛋白分离。使用ACE(®) 5 C18柱（250 mm×4.6 mm，5 μm），以磷酸盐缓冲液（pH 2.7）和乙腈（77:23，v/v）组成的等度流动相进行分离。紫外检测器设定在277 nm。该方法在0.05 - 8 μg/ml的线性范围内进行了验证，具有可接受的批间和批内精密度、准确度和稳定性。该方法简单快速，可用于定量患有外周动脉疾病患者血浆中这种广泛使用的抗生素。