Alqahtani Ali, Alqahtani Taha, Al Fatease Adel, Tolba Enas H
Department of Pharmacology, College of Pharmacy, King Khalid University, Abha, 62529, Saudi Arabia.
Department of Pharmaceutics, College of Pharmacy, King Khalid University, Abha, 62529, Saudi Arabia.
BMC Chem. 2024 Sep 16;18(1):172. doi: 10.1186/s13065-024-01286-0.
Herein, a novel UV spectrophotometric method coupled with chemometric tools was developed for the simultaneous determination of three fluoroquinolone antibiotics: ciprofloxacin, lomefloxacin, and enrofloxacin. Such integration of UV spectroscopy and chemometric analysis proved to be a simple, rapid, and cost-effective approach for the quantification of these clinically important pharmaceutical compounds and aid in their quality control analysis. The method employed firefly algorithm for variable selection and partial least squares (PLS) regression for model calibration. The developed method was validated by independent test set in addition the accuracy, intra and inter-day precision as per ICH guidelines which showed a satisfactory performance with mean recovery ranged between 98.18 and 101.83 with %RSD < 2. Besides, the developed method displayed ultrasensitive levels with LODs (0.0803, 0.1125, 0.1309 µg/mL) and LOQs (0.2434, 0.3409, 0.3968 µg/mL) for ciprofloxacin, lomefloxacin, and enrofloxacin, respectively. The greenness and blueness of the developed method were also evaluated using the recently proposed Analytical GREEnness metric approach (AGREE) and Blue applicability grade index (BAGI) tools, which showed a high AGREE score of 0.79 and a BAGI score of 77.5. These results indicate that the developed method provides an environmentally friendly alternative to the traditionally used chromatographic techniques, while maintaining high analytical practicability. Finally, the application of the developed methodology was demonstrated on real pharmaceutical and tap water samples, and the results were in good agreement with those obtained by the reference HPLC method indicating the reliability and suitability of the proposed spectrophotometric method for routine analysis of fluoroquinolone antibiotics.
在此，开发了一种结合化学计量学工具的新型紫外分光光度法，用于同时测定三种氟喹诺酮类抗生素：环丙沙星、洛美沙星和恩诺沙星。紫外光谱法与化学计量学分析的这种结合被证明是一种简单、快速且经济高效的方法，可用于定量这些临床上重要的药物化合物，并有助于其质量控制分析。该方法采用萤火虫算法进行变量选择，并采用偏最小二乘法（PLS）回归进行模型校准。除了根据ICH指南进行准确性、日内和日间精密度验证外，还通过独立测试集对所开发的方法进行了验证，结果显示性能令人满意，平均回收率在98.18%至101.83%之间，相对标准偏差（%RSD）<2。此外，所开发的方法对环丙沙星、洛美沙星和恩诺沙星分别显示出超灵敏水平，检测限（LOD）分别为0.0803、0.1125、0.1309 μg/mL，定量限（LOQ）分别为0.2434、0.3409、0.3968 μg/mL。还使用最近提出的分析绿色度指标方法（AGREE）和蓝色适用性等级指数（BAGI）工具评估了所开发方法的绿色度和蓝色度，结果显示AGREE得分为0.79，BAGI得分为77.5。这些结果表明，所开发的方法在保持高分析实用性的同时，为传统使用的色谱技术提供了一种环境友好的替代方法。最后，在所开发的方法在实际药物和自来水样品上进行了应用，结果与参考高效液相色谱法获得的结果高度一致，表明所提出的分光光度法用于氟喹诺酮类抗生素常规分析的可靠性和适用性。
