Mahdavinezhad Ali, Mousavi-Bahar Seyed Habibollah, Poorolajal Jalal, Yadegarazari Reza, Jafari Mohammad, Shabab Nooshin, Saidijam Massoud
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Urology and Nephrology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Int J Mol Cell Med. 2015 Winter;4(1):32-9.
Bladder cancer (BC) ranks the second most common genitourinary tract malignant tumor with high mortality and 70% recurrence rate worldwide. MiRNAs expression has noticeable role in bladder tumorigenesis. The purpose of this study was to assess miR-200c, miR-30b and miR-141 in tissue samples of patients with BC and healthy adjacent tissue samples and their association with muscle invasion, grade and the size of the tumor. Transurethral resection tissue samples were collected from thirty- five newly diagnosed untreated patients with BC from 2013 to 2014. The control group consisted of adjacent normal urothelium. All samples, observed by two pathologists, were diagnosed transitional cell carcinomas (TCC) with the proportion of tumor cells greater than 80%. Total RNA including miRNAs was extracted from about 50 mg tissue samples by applying TRIzol reagent. 2((-ΔΔ CT)) method was used to calculate relative quantification of miRNA expression. Two of 35 patients were females and the other 33 were males. Invasion to bladder muscle was observed in 13 (37%) cases. MiR-141, miR-200-c and miR30-b were up-regulated in 91%, 79% and 64% of malignant tissues, respectively. Down-regulation of miR-141 had a strong association with muscle invasion (P= 0.017). Significant inverse correlation between grading and miRNA-141 level was observed (P= 0.043).
膀胱癌（BC）是全球第二大常见的泌尿生殖系统恶性肿瘤，死亡率高，复发率达70%。微小RNA（miRNA）表达在膀胱肿瘤发生过程中具有显著作用。本研究旨在评估BC患者组织样本及健康相邻组织样本中的miR-200c、miR-30b和miR-141，以及它们与肌肉浸润、肿瘤分级和大小的关系。2013年至2014年，从35例新诊断的未经治疗的BC患者中收集经尿道切除组织样本。对照组为相邻正常尿路上皮。所有样本经两名病理学家观察，均诊断为移行细胞癌（TCC），肿瘤细胞比例大于80%。使用TRIzol试剂从约50mg组织样本中提取包括miRNA在内的总RNA。采用2^（-ΔΔCT）法计算miRNA表达的相对定量。35例患者中2例为女性，其余33例为男性。13例（37%）观察到膀胱肌肉浸润。miR-141、miR-200-c和miR30-b在恶性组织中的上调率分别为91%、79%和64%。miR-141的下调与肌肉浸润密切相关（P = 0.017）。观察到分级与miRNA-141水平之间存在显著负相关（P = 0.043）。
