羊水内的微小RNA miR-4535和miR-1915-5p作为绒毛膜羊膜炎的预测生物标志物
MicroRNAs miR-4535 and miR-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis.
作者信息
Kiyoshima Chihiro, Shirasu Naoto, Urushiyama Daichi, Fukagawa Satoshi, Hirakawa Toyofumi, Yoshikawa Kenichi, Izuchi Daisuke, Miyata Kohei, Kurakazu Masamitsu, Yotsumoto Fusanori, Hiromatsu Kenji, Nomiyama Makoto, Eiji Ohta, Hirose Shinichi, Ogura Yoshitoshi, Hayashi Tetsuya, Hata Kenichiro, Nabeshima Kazuki, Yasunaga Shin'ichiro, Miyamoto Shingo
机构信息
Department of Obstetrics & Gynecology, Fukuoka University, Fukuoka, Japan.
Department of Biochemistry, Fukuoka University, Fukuoka, Japan.
出版信息
Future Sci OA. 2021 Feb 15;7(5):FSO686. doi: 10.2144/fsoa-2021-0006.
BACKGROUND
This study was performed to investigate the clinical significance of miR-4535 and miR-1915-5p in severe chorioamnionitis.
MATERIALS & METHODS: Amniotic fluid samples from 37 patients with severe chorioamnionitis were subjected to miRNA array analysis and ddPCR™. Diagnostic values were assessed using the receiver operating characteristic curve. The patients were separated into three groups according to Blanc's criteria.
RESULTS
The expression of miR-4535 and miR-1915-5p was significantly correlated with the copy number of 16S rDNA, had extremely high diagnostic accuracy for severe chorioamnionitis, and was linked to maternal and fetal inflammation.
CONCLUSION
miR-4535 and miR-1915-5p serve as promising biomarkers for the diagnosis of severe chorioamnionitis.
背景
本研究旨在探讨miR - 4535和miR - 1915 - 5p在重度绒毛膜羊膜炎中的临床意义。
材料与方法
对37例重度绒毛膜羊膜炎患者的羊水样本进行miRNA芯片分析和数字滴度PCR（ddPCR™）。使用受试者工作特征曲线评估诊断价值。根据布兰克标准将患者分为三组。
结果
miR - 4535和miR - 1915 - 5p的表达与16S rDNA拷贝数显著相关，对重度绒毛膜羊膜炎具有极高的诊断准确性，并且与母婴炎症相关。
结论
miR - 4535和miR - 1915 - 5p有望成为诊断重度绒毛膜羊膜炎的生物标志物。
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