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刺榄因在体内通过激活MAPK/ERK和PI3K/AKT信号通路促进血管生成而加速伤口愈合。

Vaccarin hastens wound healing by promoting angiogenesis via activation of MAPK/ERK and PI3K/AKT signaling pathways in vivo.

作者信息

Hou Bao, Cai Weiwei, Chen Ting, Zhang Zhixuan, Gong Haifeng, Yang Wei, Qiu Liying

机构信息

Assistant Experimentalist, Department of Basic Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, China. Technical procedures, manuscript preparation.

Experimentalist, Department of Basic Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, China. Histological examinations, manuscript preparation.

出版信息

Acta Cir Bras. 2020 Feb 7;34(12):e201901202. doi: 10.1590/s0102-865020190120000002. eCollection 2020.

DOI:10.1590/s0102-865020190120000002
PMID:32049183
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7006371/
Abstract

PURPOSE

To explore the potential role and unclear molecular mechanisms of vaccarin in wound healing.

METHODS

Rats' skin excision model to study the effects of vaccarin on wound healing in vivo . Hematoxylin and eosin staining was performed to evaluate Histopathologic characteristics. Immunohistochemistry was employed to assess the effects of vaccarin in accelerating angiogenesis. Western blot was used to evaluate relative protein expressed levels.

RESULTS

Vaccarin could significantly promote wound healing and endothelial cells and fibroblasts proliferation in the wound site. Immunohistochemistry and Western blot studies showed that the nodal proteins and receptor (bFGFR) related to angiogenesis signaling pathway were activated, and the microvascular density in the wound site was markedly higher than that in the control group.

CONCLUSIONS

The present study was the first to demonstrate that vaccarin is able to induce angiogenesis and accelerate wound healing in vivo by increasing expressions of p-Akt, p-Erk and p-bFGFR. This process is mediated by MAPK/ERK and PI3K/AKT signaling pathways.

摘要

目的

探讨 vaccarin 在伤口愈合中的潜在作用及不明分子机制。

方法

采用大鼠皮肤切除模型研究 vaccarin 对体内伤口愈合的影响。进行苏木精-伊红染色以评估组织病理学特征。采用免疫组织化学法评估 vaccarin 在促进血管生成方面的作用。使用蛋白质免疫印迹法评估相关蛋白的表达水平。

结果

vaccarin 可显著促进伤口愈合以及伤口部位内皮细胞和成纤维细胞的增殖。免疫组织化学和蛋白质免疫印迹研究表明，与血管生成信号通路相关的节点蛋白和受体（bFGFR）被激活，且伤口部位的微血管密度明显高于对照组。

结论

本研究首次证明 vaccarin 能够通过增加 p-Akt、p-Erk 和 p-bFGFR 的表达来诱导血管生成并加速体内伤口愈合。这一过程由 MAPK/ERK 和 PI3K/AKT 信号通路介导。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/2bd3b28691e2/1678-2674-acb-34-12-e201901202-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/1472b1e7a1ce/1678-2674-acb-34-12-e201901202-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/97982e7c5896/1678-2674-acb-34-12-e201901202-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/a59ea4d279e0/1678-2674-acb-34-12-e201901202-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/3bb4c1d52a47/1678-2674-acb-34-12-e201901202-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/cf6150939369/1678-2674-acb-34-12-e201901202-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/a912d1f221e8/1678-2674-acb-34-12-e201901202-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/2bd3b28691e2/1678-2674-acb-34-12-e201901202-gf07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/1472b1e7a1ce/1678-2674-acb-34-12-e201901202-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/97982e7c5896/1678-2674-acb-34-12-e201901202-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/a59ea4d279e0/1678-2674-acb-34-12-e201901202-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/3bb4c1d52a47/1678-2674-acb-34-12-e201901202-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/cf6150939369/1678-2674-acb-34-12-e201901202-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/a912d1f221e8/1678-2674-acb-34-12-e201901202-gf06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7da/7006371/2bd3b28691e2/1678-2674-acb-34-12-e201901202-gf07.jpg

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