Li Ying-Qin, Tang Ying, Fu Rao, Meng Qiu-Hua, Zhou Xue, Ling Ze-Min, Cheng Xiao, Tian Su-Wei, Wang Guo-Jie, Liu Xue-Guo, Zhou Li-Hua
Department of Anatomy, Zhong Shan School of Medicine, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China.
Department of Radiology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510120, P.R. China.
Mol Med Rep. 2015 Jul;12(1):913-20. doi: 10.3892/mmr.2015.3532. Epub 2015 Mar 23.
Although studies have been undertaken on gadolinium labeling-based molecular imaging in magnetic resonance imaging (MRI), the use of non-ionic gadolinium in the tracking of stem cells remains uncommon. To investigate the efficiency in tracking of stem cells with non-ionic gadolinium as an MRI contrast agent, a rhodamine-conjugated fluorescent reagent was used to label bone marrow stromal cells (BMSCs) of neonatal rats in vitro, and MRI scanning was undertaken. The fluorescent-conjugated cell uptake reagents were able to deliver gadodiamide into BMSCs, and cell uptake was verified using flow cytometry. In addition, the labeled stem cells with paramagnetic contrast medium remained detectable by an MRI monitor for a minimum of 28 days. The present study suggested that this method can be applied efficiently and safely for the labeling and tracking of bone marrow stromal cells in neonatal rats.
尽管已经开展了关于磁共振成像（MRI）中基于钆标记的分子成像研究，但在干细胞追踪中使用非离子型钆仍然不常见。为了研究以非离子型钆作为MRI造影剂追踪干细胞的效率，使用一种罗丹明偶联的荧光试剂在体外标记新生大鼠的骨髓基质细胞（BMSCs），并进行MRI扫描。荧光偶联的细胞摄取试剂能够将钆双胺递送至BMSCs中，并使用流式细胞术验证细胞摄取情况。此外，带有顺磁性造影剂标记的干细胞在MRI监测下至少28天仍可被检测到。本研究表明，该方法可有效且安全地应用于新生大鼠骨髓基质细胞的标记和追踪。
