Cell Therapy Center, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration, Ministry of Education, Beijing 100053, China.
Sci China Life Sci. 2011 Nov;54(11):981-7. doi: 10.1007/s11427-011-4239-x. Epub 2011 Dec 16.
Recently, transplantation of allogeneic and autologous cells has been used for regenerative medicine. A critical issue is monitoring migration and homing of transplanted cells, as well as engraftment efficiency and functional capability in vivo. Monitoring of superparamagnetic iron oxide (SPIO) particles by magnetic resonance imaging (MRI) has been used in animal models and clinical settings to track labeled cells. A major limitation of MRI is that the signals do not show biological characteristics of transplanted cells in vivo. Bone marrow mesenchymal stem cells (MSCs) have been extensively investigated for their various therapeutic properties, and exhibit the potential to differentiate into cells of diverse lineages. In this study, cynomolgus monkey MSCs (cMSCs) were labeled with Molday ION Rhodamine-B™ (MIRB), a new SPIO agent, to investigate and characterize the biophysical and MRI properties of labeled cMSCs in vitro and in vivo. The results indicate that MIRB is biocompatible and useful for cMSCs labeling and cell tracking by multimodality imaging. Our method is helpful for detection of transplanted stem cells in vivo, which is required for understanding mechanisms of cell therapy.
最近，异体和自体细胞移植已被用于再生医学。一个关键问题是监测移植细胞的迁移和归巢、体内的植入效率和功能能力。超顺磁氧化铁（SPIO）颗粒的磁共振成像（MRI）监测已在动物模型和临床环境中用于跟踪标记的细胞。MRI 的一个主要限制是信号不能显示体内移植细胞的生物学特征。骨髓间充质干细胞（MSCs）因其多种治疗特性而被广泛研究，并具有分化为多种谱系细胞的潜力。在这项研究中，使用新型超顺磁氧化铁试剂 Molday ION Rhodamine-B™（MIRB）标记食蟹猴 MSCs（cMSCs），以研究和表征体外和体内标记的 cMSCs 的生物物理和 MRI 特性。结果表明，MIRB 具有生物相容性，可用于 cMSCs 标记和细胞追踪的多模态成像。我们的方法有助于检测体内移植的干细胞，这是理解细胞治疗机制所必需的。
