efimochkina N R, Bykova I B, Batishcheva S Iu, Minaeva L P, Markova Iu M, Korotkevich Iu V, Shilov G Iu, Sheveleva S A
Vopr Pitan. 2014;83(5):33-42.
Investigations of microbial contamination and species composition of the Enterobacteriaceae family in fresh vegetables and lettuce has been conducted. The objects of study were new types of fresh ready-to-eat vegetable foods - salads, sliced vegetables and mixtures thereof, sampled at the main stages of production, including washing, antimicrobial treatment with sodium hypochlorite, and packaging in the film under vacuum. Quantitative analysis of Enterobacteriaceae levels in fresh and packaged vegetables and salads showed that their part in the total amount of microbial contaminants is large enough. Average Enterobacteriaceae content ranged from 2,14 to 3,34 lg cfu/g, reaching in some samples values 4,38-4,74 lg, comparable with the levels of total bacteria. Considerable species diversity of microflora contaminating ready-to-eat vegetable products has been found. Bacteria of the genera Enterobactel; Pantoea, Citrobacter, Serratia, Pseudomonas, Kluyvera, Klebsiella, Escherichia, Rahnella, Acinetobacter were found in the salads and sliced vegetables. In the tested samples most frequently detected Enterobacter spp. - 37% of identified strains and Pantoea spp - 25% of strains. The data on the composition and levels of microbial contaminants in vegetable and salad products highlight not only the need to monitor coliform bacteria - traditional indicators of faecal contamination of raw materials, but also the need to introduce criteria for the amount of Enterobacteriaceae.
已对新鲜蔬菜和生菜中肠杆菌科的微生物污染及物种组成进行了调查。研究对象是新型即食新鲜蔬菜食品——沙拉、切片蔬菜及其混合物,在生产的主要阶段进行采样,包括清洗、用次氯酸钠进行抗菌处理以及真空薄膜包装。对新鲜和包装蔬菜及沙拉中肠杆菌科水平的定量分析表明,它们在微生物污染物总量中所占比例足够大。肠杆菌科的平均含量在2.14至3.34 lg cfu/g之间,在一些样品中达到4.38 - 4.74 lg,与总细菌水平相当。已发现污染即食蔬菜产品的微生物群落具有相当大的物种多样性。在沙拉和切片蔬菜中发现了肠杆菌属、泛菌属、柠檬酸杆菌属、沙雷氏菌属、假单胞菌属、克吕沃菌属、克雷伯菌属、大肠杆菌属、拉恩菌属、不动杆菌属的细菌。在所测试的样品中,最常检测到的是肠杆菌属菌株——占已鉴定菌株的37%,以及泛菌属菌株——占菌株的25%。蔬菜和沙拉产品中微生物污染物的组成和水平数据不仅突出了监测作为原材料粪便污染传统指标的大肠菌群的必要性,还突出了引入肠杆菌科数量标准(的必要性)。