Siriken Belgin, Türk Haldun, Yildirim Tuba, Durupinar Belma, Erol Irfan
Dept. of Water Products Diseases, Faculty of Veterinary Medicine, Ondokuz Mayis Univ, Samsun, Turkey.
Ondokuz Mayıs İlçe Gıda ve Tarım Hayvancılık Müdürlüğü, Hükümet Konağı, Ondokuz Mayıs, Samsun, Turkey.
J Food Sci. 2015 May;80(5):M1044-50. doi: 10.1111/1750-3841.12829. Epub 2015 Mar 28.
This study was conducted in a Turkish province to investigate the presence of Salmonella spp. in 150 chicken meat samples using 2 phenotyping techniques: classic culture technique (CCT) and immunomagnetic separation (IMS). For the confirmation of the isolates at molecular levels, invA gene was detected in these isolates. The presence of invA, class 1 (Cls1) integrons, and integrase (Int1) genes was demonstrated by PCR assay; and the resistance of the isolated Salmonella spp. strains to antibiotics was determined by disk diffusion test. All the cultural and PCR results were evaluated together; Salmonella spp. were detected in a total of 64 (42.66%) chicken meat samples. Contamination rate was higher in carcasses (53.33%, n = 75) than in meat pieces (32%, n = 75). When results of standard culture were compared with IMS technique, IMS (n = 54) showed a clear superiority over the CCT (n = 38). A very high resistance rate (≥ 89.28%) to vancomycin, tetracycline, streptomycin, or nalidixic acid was found. Trimethoprim-sulfamethoxazole resistance was present in 32.14%. Relatively lower incidence of resistance (≤ 8.33%) to gentamicin, chloramphenicol, ampicillin, and ceftriaxone was observed. Concurrent resistance to at least 4 antibiotics was detected in 92.85% of the isolates. Cls1 integrons and Int1 were positive in 80.95% and 95.23% of the isolates, respectively. However, Int1 alone was detected in 15.47% (n = 13). In conclusion, the high prevalence of Salmonella spp. in chicken meat may pose a potential public health risk, and the presence of antibiotic-resistant Salmonella spp. isolate together with Cls1 integron and/or integrase might play an important role in horizontal antibiotic gene transfer.
本研究在土耳其的一个省份开展，旨在使用两种表型分析技术（经典培养技术（CCT）和免疫磁珠分离法（IMS））调查150份鸡肉样本中沙门氏菌属的存在情况。为了在分子水平上确认分离株，在这些分离株中检测到了invA基因。通过聚合酶链反应（PCR）检测invA、1类（Cls1）整合子和整合酶（Int1）基因的存在；通过纸片扩散试验确定分离出的沙门氏菌属菌株对抗生素的耐药性。综合评估所有培养和PCR结果；总共在64份（42.66%）鸡肉样本中检测到沙门氏菌属。胴体的污染率（53.33%，n = 75）高于肉块（32%，n = 75）。将标准培养结果与IMS技术的结果进行比较时，IMS（n = 54）显示出明显优于CCT（n = 38）。发现对万古霉素、四环素、链霉素或萘啶酸的耐药率非常高（≥ 89.28%）。甲氧苄啶-磺胺甲恶唑耐药率为32.14%。观察到对庆大霉素、氯霉素、氨苄西林和头孢曲松的耐药发生率相对较低（≤ 8.33%）。在92.85%的分离株中检测到对至少4种抗生素的同时耐药。Cls1整合子和Int1分别在80.95%和95.23%的分离株中呈阳性。然而，仅在15.47%（n = 13）的样本中检测到Int1。总之，鸡肉中沙门氏菌属的高流行率可能构成潜在的公共卫生风险，耐抗生素的沙门氏菌属分离株与Cls1整合子和/或整合酶的存在可能在抗生素基因水平转移中起重要作用。
