George B, Alam Ch Mashhood, Kumar R Vinoth, Gnanasekaran Prabu, Chakraborty S
Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India.
Virology. 2015 Aug;482:41-50. doi: 10.1016/j.virol.2015.03.003. Epub 2015 Mar 26.
The compound microsatellites consist of two or more individual microsatellites, originate from mutation or imperfection in simple repeat sequences. The reports on systematic analysis of the occurrence, size and density of compound microsatellite (cSSR) types are very rare. Our study indicates that cSSRs are clustered at specific regions in the begomovirus genomes. cSSRs were overrepresented in majority of begomovirus genomes indicating that they might have some functional significance. Further, non-random distribution pattern of cSSR in begomovirus genomes was significantly correlated with the recombination breakpoint positions in the genome. The analysis of cSSR regions in the viral genome indicates the presence of stem loop (hairpin) secondary structure. The significance of these findings in biology of geminiviruses is discussed based on our present understanding of recombination and repetitive DNA. To our knowledge, this is the first analysis suggesting the possible association between recombination and microsatellites in any viral genome.
复合微卫星由两个或更多个单独的微卫星组成，起源于简单重复序列中的突变或缺陷。关于复合微卫星（cSSR）类型的发生、大小和密度的系统分析报告非常罕见。我们的研究表明，cSSR在双生病毒基因组的特定区域聚集。cSSR在大多数双生病毒基因组中占比过高，表明它们可能具有某些功能意义。此外，双生病毒基因组中cSSR的非随机分布模式与基因组中的重组断点位置显著相关。对病毒基因组中cSSR区域的分析表明存在茎环（发夹）二级结构。基于我们目前对重组和重复DNA的理解，讨论了这些发现在双生病毒生物学中的意义。据我们所知，这是首次分析表明在任何病毒基因组中重组与微卫星之间可能存在关联。
