Sahu Anurag Kumar, Sanan-Mishra Neeti
Plant RNAi Biology Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, 110067 India.
3 Biotech. 2020 May;10(5):235. doi: 10.1007/s13205-020-02220-6. Epub 2020 May 6.
The complete bipartite genome, consisting of DNA-A and DNA-B, of a novel begomovirus isolate associated with apical leaf curling and crinkled leaf disease of Chili () from New Delhi, India was cloned and sequenced. The sequence of DNA-A (2737 nt) and DNA-B (2692 nt) of the virus was submitted to NCBI, USA under the accessions MK069591 & MG597211, respectively. Sequence identity of the common region (CR) and presence of identical iterons (GAGTG) between the DNA-A and DNA-B clones indicate that they constitute a related pair. The virus corresponds to a novel species of tomato leaf curl virus and sequence analysis has ruled out the involvement of recombination events in its evolution. Therefore, we report the complete nucleotide sequence of a new bipartite begomovirus infecting a vegetable crop communally cultivated throughout India.
从印度新德里分离出一种与辣椒顶端叶片卷曲和皱叶病相关的新型双生病毒，其由DNA - A和DNA - B组成的完整二分体基因组被克隆并测序。该病毒的DNA - A（2737 nt）和DNA - B（2692 nt）序列分别以登录号MK069591和MG597211提交至美国国家生物技术信息中心（NCBI）。DNA - A和DNA - B克隆之间共同区域（CR）的序列同一性以及相同重复序列（GAGTG）的存在表明它们构成相关的一对。该病毒对应番茄卷叶病毒的一个新物种，序列分析排除了重组事件在其进化中的参与。因此，我们报道了一种新的二分体双生病毒的完整核苷酸序列，该病毒感染印度各地共同种植的一种蔬菜作物。
