Chrzanowska Anna M, Poliwoda Anna, Wieczorek Piotr P
Opole University, Faculty of Chemistry, Pl. Kopernika 11a, 45-040 Opole, Poland.
Opole University, Faculty of Chemistry, Pl. Kopernika 11a, 45-040 Opole, Poland.
J Chromatogr A. 2015 May 1;1392:1-9. doi: 10.1016/j.chroma.2015.03.015. Epub 2015 Mar 14.
Selective molecularly imprinted silica polymer (SiO2MIP) for extraction of biochanin A, daidzein and genistein was synthesized using the surface molecular imprinting technique with the silica gel as a support. Biochanin A (BCA) was used as a template, 3-aminopropyltriethoxysilane (APTES) as a functional monomer, and tetraethoxysilicane (TEOS) as a cross-linker. Non-imprinted polymer with the sol-gel process (SiO2NIP) was also prepared for comparison. The synthesized polymers were characterized by Fourier transform infrared spectrometry (FTIR), scanning electron microscopy (SEM) and a standard Brunauer-Emett-Teller (BET) and Barret-Joyner-Halenda (BJH) analysis. The obtained results indicated the structural differences between imprinted and non-imprinted polymers. Finally, the SiO2MIP and SiO2NIP were adopted as the adsorbents of solid phase extraction for isolation and preconcentration of biochanin A and its structural analogues-daidzein and genistein from aqueous and urine samples. The performance analysis revealed that SiO2MIP displayed better affinity to the three investigated isoflavones compared with SiO2NIP. The recoveries of spiked samples for studied analytes ranged from 65.7% to 102.6% for molecularly imprinted silica polymer and 8.9-16.0% for non-imprinted sorbents.
以硅胶为载体，采用表面分子印迹技术合成了用于提取生物活性成分A、大豆苷元和染料木黄酮的选择性分子印迹硅胶聚合物（SiO2MIP）。以生物活性成分A（BCA）为模板，3-氨丙基三乙氧基硅烷（APTES）为功能单体，四乙氧基硅烷（TEOS）为交联剂。还采用溶胶-凝胶法制备了非印迹聚合物（SiO2NIP）用于比较。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）以及标准的布鲁诺尔-埃米特-泰勒（BET）和巴雷特-乔伊纳-哈伦达（BJH）分析对合成的聚合物进行了表征。所得结果表明了印迹聚合物和非印迹聚合物之间的结构差异。最后，将SiO2MIP和SiO2NIP用作固相萃取吸附剂，从水样和尿液样本中分离和预富集生物活性成分A及其结构类似物——大豆苷元和染料木黄酮。性能分析表明，与SiO2NIP相比，SiO2MIP对所研究的三种异黄酮表现出更好的亲和力。对于加标样品，分子印迹硅胶聚合物对所研究分析物的回收率在65.7%至102.6%之间，而非印迹吸附剂的回收率为8.9 - 16.0%。
