采用定量聚合酶链反应（PCR）方法结合单叠氮溴化乙锭处理检测环境水中的军团菌属。
Detection of Legionella species in environmental water by the quantitative PCR method in combination with ethidium monoazide treatment.
作者信息
Inoue Hiroaki, Takama Tomoko, Yoshizaki Miwa, Agata Kunio
机构信息
Tsukuba Research Laboratories, Aquas Corporation.
出版信息
Biocontrol Sci. 2015;20(1):71-4. doi: 10.4265/bio.20.71.
We detected Legionella species in 111 bath water samples and 95 cooling tower water samples by using a combination of conventional plate culture, quantitative polymerase chain reaction (qPCR) and qPCR combined with ethidium monoazide treatment (EMA-qPCR) methods. In the case of bath water samples, Legionella spp. were detected in 30 samples by plate culture, in 85 samples by qPCR, and in 49 samples by EMA-qPCR. Of 81 samples determined to be Legionella-negative by plate culture, 56 and 23 samples were positive by qPCR and EMA-qPCR, respectively. Therefore, EMA treatment decreased the number of Legionella-positive bath water samples detected by qPCR. In contrast, EMA treatment had no effect on cooling tower water samples. We therefore expect that EMA-qPCR is a useful method for the rapid detection of viable Legionella spp. from bath water samples.
我们通过结合传统平板培养、定量聚合酶链反应（qPCR）以及qPCR结合单叠氮溴化乙锭处理（EMA-qPCR）方法，在111份洗浴水样和95份冷却塔水样中检测军团菌属。对于洗浴水样，通过平板培养在30份样本中检测到军团菌属，通过qPCR在85份样本中检测到，通过EMA-qPCR在49份样本中检测到。在平板培养确定为军团菌阴性的81份样本中，分别有56份和23份样本通过qPCR和EMA-qPCR检测为阳性。因此，EMA处理减少了通过qPCR检测到的军团菌阳性洗浴水样数量。相比之下，EMA处理对冷却塔水样没有影响。因此，我们预计EMA-qPCR是一种从洗浴水样中快速检测存活军团菌属的有用方法。
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