Inoue Hiroaki, Takama Tomoko, Yoshizaki Miwa, Agata Kunio
Tsukuba Research Laboratories, Aquas Corporation.
Biocontrol Sci. 2015;20(1):71-4. doi: 10.4265/bio.20.71.
We detected Legionella species in 111 bath water samples and 95 cooling tower water samples by using a combination of conventional plate culture, quantitative polymerase chain reaction (qPCR) and qPCR combined with ethidium monoazide treatment (EMA-qPCR) methods. In the case of bath water samples, Legionella spp. were detected in 30 samples by plate culture, in 85 samples by qPCR, and in 49 samples by EMA-qPCR. Of 81 samples determined to be Legionella-negative by plate culture, 56 and 23 samples were positive by qPCR and EMA-qPCR, respectively. Therefore, EMA treatment decreased the number of Legionella-positive bath water samples detected by qPCR. In contrast, EMA treatment had no effect on cooling tower water samples. We therefore expect that EMA-qPCR is a useful method for the rapid detection of viable Legionella spp. from bath water samples.
我们通过结合传统平板培养、定量聚合酶链反应（qPCR）以及qPCR结合单叠氮溴化乙锭处理（EMA-qPCR）方法，在111份洗浴水样和95份冷却塔水样中检测军团菌属。对于洗浴水样，通过平板培养在30份样本中检测到军团菌属，通过qPCR在85份样本中检测到，通过EMA-qPCR在49份样本中检测到。在平板培养确定为军团菌阴性的81份样本中，分别有56份和23份样本通过qPCR和EMA-qPCR检测为阳性。因此，EMA处理减少了通过qPCR检测到的军团菌阳性洗浴水样数量。相比之下，EMA处理对冷却塔水样没有影响。因此，我们预计EMA-qPCR是一种从洗浴水样中快速检测存活军团菌属的有用方法。
