Amlot P L, Grennan D, Humphrey J H
Eur J Immunol. 1985 May;15(5):508-12. doi: 10.1002/eji.1830150516.
The murine antibody response to T-independent (TI)-2 antigens [2,4-dinitrophenyl-Lys-Ficoll (DNP-FIC) and DNP-hydroxyethyl starch (HES)] was impaired long after splenectomy, while responses to TI-1 [trinitrophenylated lipopolysaccharide (TNP-LPS)] and thymus-dependent [TNP-keyhole limpet hemocyanin (KLH)] antigens were largely unaffected. The antibody response to these TI-2 antigens was exclusively against the conjugated epitopes [DNP-, fluorescein isothiocyanate (FITC)- or tetramethylrhodamine isothiocyanate (TRITC)-Ficoll or -HES]. Fluorescent conjugates of Ficoll and HES localize selectively to the splenic marginal zone macrophages. This localization was not affected by 750 cGy of X-irradiation, but the antibody response to the TI-2 antigens was abrogated for 14 days. Administration of spleen cells restored the antibody response to these TI-2 antigens in otherwise intact irradiated mice but not if they had been splenectomized. Our findings indicate that the antibody response to TI-2 antigens depends upon stimulation of B cells in a splenic environment. This probably involves antigen presentation by marginal zone macrophages.
脾切除术后很长一段时间内，小鼠对非胸腺依赖性（TI）-2抗原[2,4-二硝基苯基-Lys-聚蔗糖（DNP-FIC）和DNP-羟乙基淀粉（HES）]的抗体反应受损，而对TI-1[三硝基苯基化脂多糖（TNP-LPS）]和胸腺依赖性[TNP-钥孔戚血蓝蛋白（KLH）]抗原的反应基本未受影响。对这些TI-2抗原的抗体反应仅针对偶联表位[DNP-、异硫氰酸荧光素（FITC）-或四甲基异硫氰酸罗丹明（TRITC）-聚蔗糖或-羟乙基淀粉]。聚蔗糖和羟乙基淀粉的荧光偶联物选择性地定位于脾脏边缘区巨噬细胞。这种定位不受750 cGy X射线照射的影响，但对TI-2抗原的抗体反应在14天内被消除。给予脾细胞可恢复原本完整的受照射小鼠对这些TI-2抗原的抗体反应，但脾切除小鼠则不能恢复。我们的研究结果表明，对TI-2抗原的抗体反应取决于脾脏环境中B细胞的刺激。这可能涉及边缘区巨噬细胞的抗原呈递。
