Claassen E, Ott A, Boersma W J, Deen C, Schellekens M M, Dijkstra C D, Kors N, Van Rooijen N
Department of Histology, Medical Faculty, Free University, Amsterdam, The Netherlands.
Clin Exp Immunol. 1989 Sep;77(3):445-51.
Splenic tissue from mice was autotransplanted; after initial necrosis, a rapid restoration of implants into a structure histologically indistinguishable from splenic tissue was observed. The development of the marginal zone in these autotransplants, as determined with monoclonal antibodies against different splenic cell types and routine histological stains, was compared with the local and systemic response against a thymus-independent (TI) type 2 antigen. Full restoration of time course and peak of anti-trinitrophenyl (TNP) serum titres against TNP-Ficoll was observed at 4 weeks after autotransplantation. Anti-TNP antibody-forming cells were observed in subnormal and normal numbers in 2- and 4-week old autotransplants, respectively. The appearance of normal numbers of antibody-forming cells, and the restoration of antibody titres at week 4 correlated with the return of newly formed B cells in a normal marginal zone. An unexpected observation was that marginal zone macrophages did not return until 10 weeks after transplantation, thereby making the necessity for these cells in the normal TI-2 response unlikely. We conclude that normal anti-TI-2 responses (onset and peak titres) can be restored by autotransplantation of splenic tissue. B cells and marginal zone organization are responsible for this response, for which marginal zone macrophages seem expendable. The partial protection against overwhelming post-splenectomy infections, given by autotransplants, can thus be explained by restorative capabilities of these implants on antigen presentation and antibody formation against TI-2 antigens, and not by an increase (compared with splenectomized individuals) of phagocytosis by marginal zone macrophages.
将小鼠的脾脏组织进行自体移植；在最初的坏死之后，观察到植入物迅速恢复为在组织学上与脾脏组织无法区分的结构。利用针对不同脾脏细胞类型的单克隆抗体和常规组织学染色确定这些自体移植中边缘区的发育，并将其与针对非胸腺依赖性（TI）2型抗原的局部和全身反应进行比较。在自体移植后4周观察到针对TNP-Ficoll的抗三硝基苯（TNP）血清滴度的时间进程和峰值完全恢复。在2周龄和4周龄的自体移植中，分别观察到数量低于正常和正常数量的抗TNP抗体形成细胞。在第4周时，抗体形成细胞数量恢复正常以及抗体滴度恢复，这与正常边缘区中新形成的B细胞的回归相关。一个意外的观察结果是，边缘区巨噬细胞直到移植后10周才恢复，因此这些细胞在正常TI-2反应中的必要性似乎不大。我们得出结论，脾脏组织的自体移植可以恢复正常的抗TI-2反应（起始和峰值滴度）。B细胞和边缘区组织负责这种反应，而边缘区巨噬细胞似乎是多余的。因此，自体移植对脾切除术后严重感染的部分保护作用，可以通过这些植入物在抗原呈递和针对TI-2抗原的抗体形成方面的恢复能力来解释，而不是通过（与脾切除个体相比）边缘区巨噬细胞吞噬作用的增加来解释。
