Laboratory of Renal Lithiasis Research, Faculty of Sciences, University Institute of Health Sciences Research, University of Balearic Islands, Palma de Mallorca, Spain.
Laboratory of Renal Lithiasis Research, Faculty of Sciences, University Institute of Health Sciences Research, University of Balearic Islands, Palma de Mallorca, Spain.
J Urol. 2015 Sep;194(3):812-9. doi: 10.1016/j.juro.2015.03.099. Epub 2015 Mar 26.
The main aim of the current study was to evaluate the effectiveness of mixtures of magnesium, citrate and phytate as calcium oxalate crystallization inhibitors.
A turbidimetric assay in synthetic urine was performed to obtain induction times for calcium oxalate crystallization in the absence and presence of different mixtures of inhibitors. The morphology of calcium oxalate crystals in the absence or presence of inhibitors and mixtures of the inhibitors was evaluated in 2 crystallization experiments at low and high calcium oxalate supersaturation. The crystals formed were examined using scanning electron microscopy.
Examination of crystallization induction times revealed clear inhibitory effects of magnesium, citrate and phytate on calcium oxalate crystallization, supporting usefulness in the treatment and prevention of calcium oxalate nephrolithiasis. Significant synergistic effects between magnesium and phytate were observed. Scanning electron microscopy images revealed that phytate is a powerful crystal growth inhibitor of calcium oxalate, totally preventing the formation of trihydrate and monohydrate. In addition to crystallization inhibition capacity, citrate and magnesium avoided calcium oxalate crystallization by decreasing its supersaturation.
The synergistic effect between magnesium and phytate on calcium oxalate crystallization suggests that a combination of these 2 compounds may be highly useful as antilithiasis therapy.
本研究的主要目的是评估镁、柠檬酸盐和植酸盐混合物作为草酸钙结晶抑制剂的效果。
在合成尿液中进行了浊度测定，以获得草酸钙在无抑制剂和不同抑制剂混合物存在下结晶的诱导时间。在低和高草酸钙过饱和度下的 2 个结晶实验中，评估了草酸钙晶体在无抑制剂和抑制剂混合物存在下的形态。使用扫描电子显微镜检查形成的晶体。
结晶诱导时间的检查显示镁、柠檬酸盐和植酸盐对草酸钙结晶有明显的抑制作用，支持其在治疗和预防草酸钙肾结石中的应用。镁和植酸盐之间观察到显著的协同作用。扫描电子显微镜图像显示植酸盐是草酸钙的强大晶体生长抑制剂，完全阻止三水合物和一水合物的形成。除了结晶抑制能力外，柠檬酸盐和镁通过降低其过饱和度来避免草酸钙结晶。
镁和植酸盐对草酸钙结晶的协同作用表明，这两种化合物的组合可能非常有用作为抗结石治疗。
