Testa U, Vainchenker W, Beuzard Y, Rouyer-Fessard P, Guerrasio A, Titeux M, Lapotre P, Bouguet J, Breton-Gorius J, Rosa J
Eur J Biochem. 1982 Jan;121(3):649-55. doi: 10.1111/j.1432-1033.1982.tb05835.x.
The K562 cell line was cloned by dilution and the pattern of hemoglobin (Hb) production was analyzed in the clones thus obtained. The pattern of hemoglobin synthesis was different from one clone to another. According to the Hb phenotype the clones were classified in three main groups; group I no detectable Hb; group II Hb Portland; group III Hb Portland + Hb Gower I. The addition of hemin induced a better hemoglobinization and a shift in the pattern of Hb production: clones of group I were induced to produce Hb Portland and clones of group II to synthesize Hb Gower I. A constant order in the sequential expression of the different hemoglobins was observed: no Hb leads to Hb Portland + Hb Gower I. The proportion of the different globin chains varied from one clone to another, but an inverse correlation between the synthesis of G gamma and epsilon chains was observed. The alpha/non-alpha chain ratio was unbalanced in all the clones and the addition of hemin induced only a moderate increase of the synthesis of alpha chains. Recloning of three primary clones increased the homogeneity of the hemoglobin pattern, in particular after hemin induction.
通过稀释法克隆K562细胞系，并对由此获得的克隆进行血红蛋白（Hb）产生模式的分析。不同克隆之间的血红蛋白合成模式各不相同。根据Hb表型，克隆被分为三个主要组：I组，未检测到Hb；II组，波特兰血红蛋白（Hb Portland）；III组，波特兰血红蛋白 + 戈埃尔血红蛋白I（Hb Gower I）。加入血红素可诱导更好的血红蛋白化，并使Hb产生模式发生转变：I组克隆被诱导产生波特兰血红蛋白，II组克隆则合成戈埃尔血红蛋白I。观察到不同血红蛋白的顺序表达存在固定顺序：无Hb导致波特兰血红蛋白 + 戈埃尔血红蛋白I。不同珠蛋白链的比例因克隆而异，但观察到Gγ链和ε链的合成呈负相关。所有克隆中的α/非α链比例均失衡，加入血红素仅诱导α链合成适度增加。对三个原始克隆进行再克隆增加了血红蛋白模式的同质性，尤其是在血红素诱导后。
